Bioprocessing And Nano-Enzyme Formulation Facility (BANEFF)

Institut national de la recherche scientifique (INRS), Québec, Québec
Area(s) of Expertise

BANEFF works in close collaboration with universities and the industrial sector for the testing and commercialization of different technologies of bioproduction, such as enzymes, biohydrogen, butanol, citric acid and fumaric acid. In addition to the regular laboratory equipment for specific uses and with supportive analytical facilities, BANEFF comprises principally three phases: fermentation, extraction and formulation. The facilities are accessible to universities, industries and government laboratories. The three-phase installations comprise a liquid-state fermenter, two solid-state fermenters, lyophilizer, incubator shaker, ultrafiltration system and nanospray dryer, along with spectrophotometer, autoclave, nanosizer and allied equipment. The installations comprise a platform for upstream bioprocessing, downstream bioprocessing and peripheral analytical equipment. There are different ways in which clients can use the BANEFF infrastructure: (1) getting complete training on the equipment and using it themselves in the laboratory; (2) through research contracts with INRS-ETE; and (3) enhancing the research and testing potential by collaborating with the professor through different research-grant mechanisms or direct research contracts.

To date, BANEFF has carried out the diverse activities through direct training and industry-university research collaborations. BANEFF has also worked in partnership to produce enzymes for the brewery industry, biopesticides, value addition of pomace into diverse enzymes, antioxidants and animal feed, enzymes for lipid extraction and green-food alternatives.

What the facility does

Bioprocessing and nanoformulations to produce high-value and safe bioproducts

Research Services

Fermentation: solid and liquid state, pre-treatment of wastes, screening of strains for high-performance production of bioproducts, extraction and purification of bioproducts, microformulation and nanoformulation of bioproducts, analysis of unknown metabolites, degradation by-products of organic compounds in the environment for complex matrices such as wastes, waste water, sludge and soils

Sectors of Application
  • Agriculture, animal science and food
  • Energy (renewable and fossil)
  • Environmental technologies and related services

Name of specialized lab

Name of equipment

Description of function

Bioprocessing Laboratory

Liquid-state fermenter, including hydrogen and CO2 sensor and mechanical foam breaker and chiller units

For fermentation of different substrates coupled with the measurement of gases during aerobic and anaerobic fermentation

 

Solid-state fermenter (rotating drum type), including in situ sterilization, measurement of moisture, pH and CO2

For fermentation of solid and semi-solid substrates with total solids concentration exceeding 80 g/L

 

Autoclave for dry and liquid samples

Sterilization of dry and wet media for pure-strain fermentation and decontamination of petris/wastes

 

Portable benchtop laminar-flow chamber

For strain isolation, plating and all microbiological assays

 

Ultrasonication equipment

For pre-treatment and breakdown of complex organic matter

 

Phase contrast microscope

To assess contamination during fermentation and for general observation of microbial growth on petris

 

Peristaltic pumps

For transfer of different substrates and fluids into the fermenter

 

Nitrogen evaporator

To concentrate and dry precious bioproduct samples under nitrogen

Downstream Bioprocessing Laboratory

Ultrafiltration equipment

For extraction and concentration of different components of fermentation broths

 

Ultrasonication

For extraction of intracellular material

 

Lyophilization

Freeze-drying of liquid samples for pre-formulations, analysis and storage

 

SDS-PAGE

For data profiling of different protein components in the extracted fermentation broths

 

Microfiltration

For filtration of different fermented broths for analysis

 

Nanospray dryer

For nanoformulation of different types, such as encapsulation, powder and emulsion

Peripheral Analytical Laboratory

UV-Vis spectrophotometer, including microplate reader

For analysis of microbial growth, enzymes and chemicals

 

Nanosizer, including autotitration and zeta potential measurements

For analysis of particle size in the nanometer range

 

Water bath for incubation experiments during analysis

To facilitate reactions during analysis

 

Accelerated extraction system, including high pressure

For extraction of organic compounds from complex matrices, such as waste water, wastes and soils

 

Microwave extraction

For extraction of organic compounds in small volumes from solid and liquid media

  • BANEFF has worked with more than 15 industries including:
  • La Barberie Inc.
  • Lallemand Inc.
  • Magnus Inc.
  • and Aliments Breton Inc.

Title

URL

Le fabuleux destin des déchets

http://www.planete.inrs.ca/biovalorisation

Révolution dans la charcuterie : des épices comme le cumin remplacent les nitrites

http://www.radio-canada.ca/emissions/bien_dans_son_assiette/2012-2013/chronique.asp?idChronique=290…

Des épices pour remplacer les nitrites dans les charcuteries, quelle bonne idée!

http://www.ete.inrs.ca/actualites/des-epices-pour-remplacer-les-nitrites-dans-les-charcuteries-quel…