Supports high-throughput genomics projects including those requiring DNA sequencing, next generation sequencing, single cell analysis, and cell sorting.
StemCore Laboratories operates with a dual mandate: to support high-throughput “omics” projects and to provide research-enabling technology to the local scientific community. StemCore is a Platform Affiliate of the Ontario Genomics Institute and is committed to the values of research excellence, collaboration, trainee education, and innovation. Our vision is to build maintain a stable infrastructure for genomics research by seeking out projects that are challenging, cutting-edge, extend the boundaries of biological knowledge, and will positively impact human health. We actively engage in and are highly supportive of grant-writing activities aimed at securing funding for high-throughput genomic studies utilizing the advanced genomics technologies present within our laboratory (DNA sequencing, next generation sequencing, and single cell analysis). We will work with you to provide letters of support and proof-of-concept data and will participate in collaborative grant writing upon request.
- Next Generation Sequencing (RNA-Seq, Small RNA-Seq, DNA-Seq, ChIP-Seq, Methyl-Seq, Targeted Resequencing)
- Sanger DNA Sequencing
- Single Cell Analysis (10x Genomics, Fluidigm C1)
- Genotyping
- SNP Analysis
- Bisulfite Sequencing
- Cell sorting
- Flow cytometry
- Agriculture, animal science and food
- Education
- Environmental technologies and related services
- Fisheries and aquaculture
- Forestry and forest-based industries
- Healthcare and social services
- Life sciences, pharmaceuticals and medical equipment
- Manufacturing and processing
Specialized labs and equipment
| Specialized lab | Equipment | Function |
|---|---|---|
| Next Generation Sequencing Facility | Illumina MiniSeq Sequencing System | Small-scale next generation sequencing platform useful for amplicon, targeted RNA, small RNA, targeted gene panels, and small genome sequencing. Maximum of 25M reads per run @2x150 bases. |
| Illumina NextSeq 500 Whole-Genome Sequencing System | Mid-scale next generation sequencing platform useful for exome, transcriptome, ChIP-Seq, and targeted resequencing. Maximum of 400M reads per run @2x150 bases. | |
| Stratagene MX3005P Quantitative PCR System | qPCR= quantitative polymerase chain reaction. For orthogonal validation of high-throughput gene expression data and quantitation of Next Generation Sequencing libraries. | |
| Single Cell Analysis Facility | Fluidigm C1 Single Cell Analysis System | Isolates and prepares individual cells for genomic analyses including DNA, transcriptome, targeted gene expression, and miRNA sequencing. Captures, washes, stains, lyses, amplifies, and harvests cDNA from 96 cells in <13 hours for gene expression analysis. |
| Fluidigm Biomark HD PCR system | Performs high-throughput nanofluidics genomics analysis of single cells or bulk populations. | |
| 10x Genomics instrument | Single Cell Analysis for transcriptome, immunotherapy, and other applications. | |
| AATI Fragment Analyzer | Qualitive assessment of DNA, RNA, and protein. | |
| Thermo Fisher Scientific Qubit 3.0 Fluorometer | Quantitative Assessment of nucleic acids and protein. | |
| DNA Sequencing Facility | Applied Biosystems 3730 DNA Analyzer | Medium throughput capillary-based fluorescent DNA sequencing/genotyping platform. |
| MJ Research Tetrad Thermocycler | For nucleic acid amplification or other enzymatic manipulations. | |
| Cell Sorting Facility | BD MoFlo Legacy cell sorter | Lasers: 355 nm, 405 nm, 488 nm, 561nm and 640 nm. |
Additional information
| Title | URL |
|---|---|
| Ontario Genomics Institute | https://www.ontariogenomics.ca/resources/ |