StemCore Laboratories

The Ottawa Hospital, Ottawa, Ontario
Area(s) of Expertise
  • Next Generation Sequencing

    • RNA-Seq
    • Small RNA-Seq
    • DNA-Seq
    • ChIP-Seq
    • Methylation Analysis
    • Targeted Resequencing
  • Affymetrix GeneChip Analysis
  • Sanger DNA Sequencing
  • Single Cell Analysis
  • Genotyping
  • SNP Analysis
  • Multipex Ligation-dependent Probe Amplification (MLPA)
  • Bisulfite Sequencing
  • Cell sorting
  • Flow cytometry
  • qPCR
What the facility does

StemCore Laboratories operates with a dual mandate: to support high-throughput “omics” projects and to provide research-enabling technology to the local scientific community. Over the past decade, our user base has grown substantially. We now provide service to several hundred individual labs mainly in North America including those from academic institutions, government, NGO and the private sector. StemCore is a Platform Affiliate (http://www.ontariogenomics.ca/business-development/ontario-genomics-platform-affiliates-program) of the Ontario Genomics Institute. StemCore is committed to the values of research excellence, collaboration, trainee education, and innovation. Our vision is to build a stable infrastructure for genomics research by seeking out projects that are challenging, cutting-edge, extend the boundaries of biological knowledge, and will positively impact human health. Several large-scale high-throughput Genome Canada projects, such as the Stem Cell Genomics Project, have been conducted within our facilities. We also actively engage in and are highly supportive of grant-writing activities aimed at securing funding for high-throughput genomic studies utilizing the advanced genomics technologies present within our laboratory. We will work with you to provide letters of support and proof-of-concept data and will participate in collaborative grant writing upon request. Please consult with StemCore’s director, Dr. Pearl Campbell, for further details.

Research Services

Affymetrix GeneChip, Sanger DNA sequencing, Genotyping, Next-generation sequencing, Cell sorting, Flow cytometry, qPCR

Sectors of Application
  • Agriculture, animal science and food
  • Education
  • Environmental technologies and related services
  • Fisheries and aquaculture
  • Forestry and forest-based industries
  • Health care and social services
  • Life sciences, pharmaceuticals and medical equipment
  • Manufacturing and processing

Name of specialized lab

Name of equipment

Description of function

Next Generation Sequencing Facility

Illumina NeoPrep

Automated library preparation including TruSeq Stranded mRNA and TruSeq Nano DNA Prep for use on any Illumina sequencing platform

 

Illumina MiniSeq

Small-scale next generation sequencing platform useful for amplicon, targeted RNA, small RNA, targeted gene panels, and small genome sequencing. Maximum of 25M reads per run @2x150 bases

 

Illumina NextSeq 500

Mid-scale next generation sequencing platform useful for exome, transcriptome, ChIP-Seq, and targeted resequencing. Maximum of 400M reads per run @2x150 bases

 

MX 3005p (Stratagene)

Quantitative Real-time PCR instrument used for orthoganol validation of high-throughput gene expression data and quantitation of Next Generation Sequencing libraries

Single Cell Analysis Facility

Fluidigm C1 Single Cell Analysis System

Isolates and prepares individual cells for genomic analyses including DNA, transcriptome, targeted gene expression, and miRNA sequencing. Captures, washes, stains, lyses, amplifies, and harvests cDNA from 96 cells in <13 hours for gene expression analysis

 

Fluidigm Biomark HD

Performs high-throughput nanofluidics genomics analysis of single cells or bulk populations

Microarray Facility

GeneChIP System (Affymetrix)

Hybridization station, fluidics station 450 and GeneChip 7G scanner

 

AATI Fragment Analyzer (Advanced Analytical Technologis)

Qualitive assessment of DNA, RNA, and protein

 

Qubit 3.0 Fluorometer (ThermoFisher Scientific)

Quantitative Assessment of nucleic acids and protein

DNA Sequencing Facility

3730 DNA Analyzer (Applied Biosystems)

Medium throughput capillary-based fluorescent DNA sequencing/genotyping platform.

 

Tetrad (MJ Research)

Thermocycler used for nucleic acid amplification or other enzymatic manipulations

Cell Sorting Facility

MoFlo Legacy

Lasers: 355 nm, 405 nm, 488 nm, 561nm and 640 nm