Human Pluripotent Stem Cell Facility (HPSCF)

The Ottawa Hospital, Ottawa, Ontario
What the facility does

Provides training and support to research with human embryonic and induced pluripotent stem cells (iPSC)

Areas of expertise

The Human Pluripotent Stem Cell Facility is multidimensional in its reach within the scientific community. As a facility we provide:

  • All the necessary infrastructure to work with and generated human pluripotent stem cell lines
  • Training on how to work with human embryonic stem cells (hESCs)/induced pluripotent stem cells (iPSCs)
  • Troubleshooting resources for members of the community working with stem cells
  • Tools for new and experienced investigators embarking on using stem cell models
  • Contract work for researchers at the University of Ottawa, its affiliates, government agencies, and other academic and private institutions
  • Symposiums and workshops for trainee education
  • Letters of support, budgets, and collaborative grant writing upon request
Research services
  • Training on human embryonic stem cells (hESCs)/induced pluripotent stem cells (iPSCs) culture methods (harvesting, passaging, thawing and freezing)
  • Training for reprogramming (differentiation, characterization)
  • Perform as a service (cell culture, reprogramming, differentiation, characterization, CRISPR genome editing)
  • Fibroblast extraction and expansion
  • Teratoma injection and extraction
  • Protocol development
Sectors of application
  • Healthcare and social services
  • Life sciences, pharmaceuticals and medical equipment

Equipment

Function

Tri-gas incubators

For culturing cells in normoxic and hypoxic conditions

Picking hoods

For manual removal of differentiated hESCs/iPSCs and isolation of clones

Bio-cool

Control rate freezer

Lonza 4D-Nucleofector Core and X Unit

Transfection system used for episomal reprogramming and gene editing

Covaris

DNA shearing device

ProteinSimple NanoPro 1000

Proteomic analyses of small numbers of cells

Amnis ImageStream X multispectral imaging flow cytometer

High throughput fluorescence imaging of cells in suspension
  • University of Ottawa
  • Ottawa Hospital Research Institute (Core Facility Reserve Fund)
  • University of Ottawa Faculty of Medicine

Title

URL
Reprogramming progeria fibroblasts re-establishes a normal epigenetic landscape

https://www.ncbi.nlm.nih.gov/pubmed/28597562
Human Pluripotent Stem Cell-Derived TSC2 - Haploinsufficient Smooth Muscle Cells Recapitulate Features of Lymphangioleiomyomatosis

http://cancerres.aacrjournals.org/content/77/20/5491.long

Metformin Preconditioning of Human Induced Pluripotent Stem Cell-Derived Neural Stem Cells Promotes Their Engraftment and Improves Post-Stroke Regeneration and Recovery

https://www.liebertpub.com/doi/abs/10.1089/scd.2018.0055?rfr_dat=cr_pub%3Dpubmed&url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&journalCode=scd
Engineering PTEN-L for Cell-Mediated Delivery

https://www.sciencedirect.com/science/article/pii/S2329050117301225?via%3Dihub
Human induced pluripotent stem cell-derived lung progenitor and alveolar epithelial cells attenuate hyperoxia-induced lung injury

https://www.sciencedirect.com/science/article/pii/S1465324917307028?via%3Dihub