Advanced BioImaging Facility (ABIF)

McGill University, Montréal, Quebec
Area(s) of Expertise

The ABIF offers more than a dozen state-of-the-art light microscopy platforms and the training and expertise required to use the equipment to its full potential. In-depth consultations, ongoing support, image processing, image analysis and preparation of data for publication all contribute to using researchers’ time effectively, high-quality data and elevating research projects to the next level. The facility has expertise in many areas of cellular imaging, including, but not limited to, live cell imaging, FRAP, FLIM, FRET, FCS, image-correlation spectroscopy, TIRF, spectral imaging, high-content screening, multi-photon imaging, laser-capture micro-dissection, cellular-image analysis and custom-image analysis.

What the facility does

Optical microscopy, confocal, spectral, TIRF, live cell, HCS, FLIM, FRET

Research Services

Free consultations, one-on-one training, confocal microscopy, FLIM, wide-field microscopy, deconvolution, live cell time lapse, multi-colour imaging, spectral imaging, tiling for high-resolution imaging of large samples, workshops, courses, tutorials, product information sessions, equipment demonstrations

Sectors of Application
  • Life sciences, pharmaceuticals and medical equipment

Name of specialized lab

Name of equipment

Description of function

Advanced BioImaging Facility

LSM710 confocal

Spectral imaging, FRAP, raster image correlation spectroscopy (RICS), FRET, tiling, multi-position live cell time lapse

 

LSM710-FLIM confocal

Time-correlated photon-counting fluorescence lifetime imaging microscope

 

High-content screening (HCS)

Live cell time lapse combined with up to five-colour wide-field imaging. From microscope slides to 384-well plate formats. Algorithms for cell cycle, translocation, vesicle counting, cell scoring, autophagy, cell health, live/dead cell assays, mitotic index, neurite outgrowth, database and file server.

 

Multi-photon confocal microscope

LSM710 upright confocal. Integrated electrophysiology. Uncaging, second harmonic generation imaging, multi-colour simultaneous imaging.

 

LSM510 confocal

Fluorescence correlation spectroscopy (FCS)

 

Laser-capture micro-dissection

UV-laser-based laser micro-dissection and capture microscope

 

Total internal reflection fluorescence (TIRF) microscope

Live cell and image correlation TIRF microscopy