Cell Biology & Image Acquisition (CB&IA) Core Facility

University of Ottawa, Ottawa, Ontario
Area(s) of Expertise

The CB&IA Core Facility provides state-of-the-art advanced microscopes, image-analysis tools and technical support to facilitate your microscopy research. We offer consultations to determine the most appropriate microscope for your specific needs and the proper design of your experimental set-up. In addition, we provide comprehensive training and follow-up sessions for all first-time users, ensuring optimal use of the imaging systems.

What the facility does

Provides state-of-the-art advanced microscopes, image-analysis tools and technical support to facilitate microscopy-based research

Research Services

Live-cell imaging, confocal microscopy, fluorescent microscopy, wide-field microscopy, super-resolution imaging, image analysis.

Sectors of Application
  • Environmental technologies and related services
  • Life sciences, pharmaceuticals and medical equipment

Name of specialized lab

Name of equipment

Description of function

Cell Biology & Image Acquisition

Leica TCS SP5-STED confocal microscope

Confocal optical sectioning, co-localization analysis, Z-stack, 3-D projection, epifluorescence/DIC for ocular viewing, super-resolution imaging

 

Zeiss LSM 510 AxioImager M1 confocal microscope

Confocal optical sectioning, FRAP, FLIP, FRET, co-localization analysis, Z-stack, 3-D projection, tile-scan, epifluorescence/ brightfield/phase contrast/DIC for ocular viewing

 

Zeiss LSM 510 AxioImager META confocal microscope

Confocal optical sectioning, FRAP, FLIP, FRET, photoactivation/conversion, co-localization analysis, Z-stack, 3D projection, time-lapse, long-term live-cell imaging, DIC with confocal acquisition Spectral detection.

Lasers:

Diode (405 nm) 
Argon (458, 477, 488, 514 nm) 
HeNe (543 nm) 
HeNe (633 nm)

 

Zeiss LSM 800 confocal microscope

Confocal optical sectioning, FRAP, FLIP, FRET, co-localization analysis, Z-stack, 3-D projection, tile-scanning, live-imaging. Diode lasers: 405nm, 488nm, 561nm and 640nm

 

Zeiss LSM880 with Airyscan FAST-confocal microscope

Confocal optical sectioning, FRAP, FLIP, FRET, co-localization analysis, Z-stack, 3-D projection, spectral detection, Super-Resolution detector: AiryScan, suitable for live imaging when coupled with the FAST module. Diode Lasers: 405nm, 561nm, and 639nm. Argon laser: 458nm, 488nm, 514nm