Centre for Chemical Analysis, Research and Training (C-CART) – CREAIT Network

Memorial University of Newfoundland, St. John's, Newfoundland and Labrador
Area(s) of Expertise

C-CART provides research support, training and chemical analysis services to undergraduate and graduate students, university researchers, government agencies and private-sector R&D companies. The instruments associated with C-CART are grouped into five service areas: nuclear magnetic resonance (NMR) spectroscopy, X-ray crystallography (XRD), mass spectrometry, materials characterization and optical spectroscopy.

What the facility does

Research support, training, student education, chemical analysis services

Research Services

Solution and solid-state NMR spectroscopy, mass spectrometry, single-crystal XRD, optical spectroscopy, materials characterization, chemical analysis, research supportand training and education

Sectors of Application
  • Chemical industries
  • Clean technology
  • Education
  • Energy (renewable and fossil)
  • Environmental technologies and related services
  • Fisheries and aquaculture
  • Forestry and forest-based industries
  • Life sciences, pharmaceuticals and medical equipment
  • Ocean industries

Name of specialized lab

Name of equipment

Description of function

Mass Spectrometry Facility

Agilent 1100 Series LC/MSD (quadrupole)

HPLC interfaced with a single quadrupole mass analyzer (50-3000 Da mass range) and equipped with a vacuum degasser, binary pump, autosampler and UV-Vis diode array detector. ESI and APCI capable. SIM or scan modes, with positive/negative polarity ion detection. Deconvolution software for protein/peptide determination available.

  Agilent 6200 Series Accurate-Mass TOF LC/MSD HPLC ( 1260 Infinity) interfaced to a 6230 TOF MS (an orthogonal time-of-flight analyzer). HPLC equipped with vacuum degasser, quaternary pump, autosampler and variable wavelength detector (190-600nm). MS has Dual Electrospray (dual-ESI) and Atmospheric Pressure Photoionization (APPI) chambers. Ion detection in positive, negative and fast polarity switching modes.

 

Agilent 1100 Series LC/MSD (ion trap)

HPLC interfaced with an ion-trap mass analyzer (200-4000 Da extended mass range with normal resolution; 50-2200 Da standard mass range with normal and enhanced resolution) and equipped with a vacuum degasser, quaternary pump, thermostatted autosampler and UV-Vis diode array detector. ESI and APCI capable. Scan, MRM, MS(n) modes, with positive/negative/alternating polarity ion detection. Sample injection via LC or syringe pump.

 

Agilent GC-MS (6890N GC/5973 MSD)

GC interfaced to a single quadrupole MSD (1.6-800 Da mass range). SIM and scan mode with positive ion detection.

 

Waters Micromass GCT premier high-resolution MSD

MS has a high-performance, orthogonal acceleration time-of-flight (oa-TOF) mass analyzer that allows fast acquisition of full spectra with high sensitivity, mass accuracy and resolution. Mass range of 4000 Da, with accuracy between measured and calculated mass of <1mDa for measurements below m/z 200 and <5ppm for measurements above m/z 200.

Sample introduction via GC or direct insertion (DI) probe for volatile and non-volatile compounds, respectively. EI and CI modes with positive/negative polarity ion detection.

 

Applied Biosystems/MDS SCIEX 4800 matrix-assisted laser desorption/ionization (MALDI) tandem time-of-flight (TOF/TOF) spectrometer

MALDI with tandem time-of-flight analyzers. Variable ion path length to accommodate MS linear, MS reflector and MS/MS modes, with positive/negative ion polarity detection. High-resolution precursor selection (MS/MS mode).

NMR Spectroscopy Facility

Bruker AVANCE 500 MHz nuclear magnetic resonance (NMR) spectrometer

Walk-up service. TXI inverse triple resonance probe, Z-gradient for 1H observation with 13C, 15N decoupling. All standard 1D and 2D gradient experiments available. Variable temperature capable.

 

Bruker AVANCE II 600 MHz NMR

Multiple probes for high-resolution, solid-state and microimaging capabilities, enabling a wide range of materials to be researched, from proteins and inorganic solids characterization to diffusion coefficients measurements. Variable temperature capable.

 

Bruker AVANCE III 300 MHz NMR

Walk-up service. Handles solution phase samples by autosampler, with multiple nuclei (1H, 13C, 19F, 31P, 29Si, 27Al,15N, 7Li, 11B) and pulse profiles. All standard 1D and 2D gradient experiments available. Variable temperature capable.

Single-Crystal XRD Facility

Rigaku AFC8-Saturn 70 single-crystal X-ray diffractometer (XRD)

Single-crystal structure determination. Equipped with an X-Stream 2000 low-temperature system and a SHINE optic. Access to the Cambridge Structural Database.

Materials Characterization Facility

Ambios Qscope Nomad 250 atomic force microscope (AFM)

Measures up to a 40 μm2 area. Contact and non-contact modes. Analog PID feedback loop provides accurate, fast sampling frequency.

 

Cary 6000i UV-Vis-NIR spectrophotometer

High-performance dual-beam UV-Vis-NIR spectrophotometer, with a 175nm to 1800nm and A > 8 a.u. detection range by InGaAs detector. Variable slit widths (to 0.01nm). Diffuse reflectance array (DRA) accessory available.

 

Mettler-Toledo DSC1 differential scanning calorimeter (DSC)

Manual and automatic measurement of enthalpic changes from –70 oC to 550oC, with heating/cooling rates of 0.02-300K/min and 0.002-50K/min, respectively, by a high-sensitivity FRS 5 56-thermocouple sensor, Julabo intercooler and corrosion-free Ag furnace. Mass-flow controller allows for automated control of sample atmosphere through selection of various gases and variable flow rates.

 

Bruker Tensor27 FT-IR

Spectral range of 7500 to 370 cm-1, with a Pike Technologies ZnSe MIRacle ATR for direct analysis of solids, liquids, powders and gases.

 

TA Instruments Q500 thermogravimetric analyzer (TGA)

Features a high-sensitivity thermobalance (0.1μg sensitivity, ≤ ±0.1% accuracy) and a responsive low-mass furnace (ambient to 1000oC range, 0.01oC -100oC/min heating rate), with equipped hi-res TGA and modulated TGA accessories to allow for a variety of isothermal, constant heating rate and variable heating rate experiments in air or in an O2-free environment.

Optical Spectroscopy Facility

Agilent HP8453A UV-Vis diode array spectrophotometer

Single-beam spectrophotometer, 190nm to 1100nm range with 1nm slit width, <± 0.5nm wavelength accuracy, 0.02nm resolution. Features a very short acquisition time for analyzing solution and thin-film samples.

 

Photon Technology QuantaMaster 6000 spectrofluorometer

Steady-state emission and excitation spectra, time-resolved emission and synchronous scan spectra of solution and solid-phase samples. 250nm to 800nm corrected wavelength range, 185nm to 900nm uncorrected. ±0.5nm wavelength accuracy, 0.06nm resolution. Adjustable, pivoting mounting pedestal for flat solids and films. Low temperatures (77 K) available.

 

Photon Technology LaserStrobe TM-3 fluorescence lifetime spectrofluorometer

Luminescence decay measurements of solution and solid-phase samples. Features a PTI GL-3300 N2 laser (337.2nm, 800 ps fwhm) as primary excitation source, with secondary excitation wavelengths available via PTI GL-302 dye laser module (400nm, 446nm and 500nm dyes on hand; others may be available upon advance request). Low temperatures (77 K) available.

 

Applied Photophysics LKS 60 laser flash photolysis spectrometer

Transient absorption/emission decay traces and spectra of solution and solid-phase samples. 532nm, 355nm and 266nm excitation wavelengths available for crossed-beam or collinear excitation alignments. Measures kinetics over nanosecond to submillisecond time scales. Diffuse reflectance accessory (DRA) for measurement of opaque solids/films and low temperatures (77 K) available.

  • Toms of Maine
  • College of the North Atlantic
  • Centre for Aquaculture and Seafood Development Fisheries
  • Eastern Health
  • The University of British Columbia
  • Fisheries and Oceans Canada