StemCore Laboratories

The Ottawa Hospital, Ottawa, Ontario
What the facility does

Supports high-throughput genomics projects including those requiring DNA sequencing, next generation sequencing, single cell analysis, and cell sorting.

Area(s) of Expertise

StemCore Laboratories operates with a dual mandate: to support high-throughput “omics” projects and to provide research-enabling technology to the local scientific community. StemCore is a Platform Affiliate of the Ontario Genomics Institute and is committed to the values of research excellence, collaboration, trainee education, and innovation. Our vision is to build maintain a stable infrastructure for genomics research by seeking out projects that are challenging, cutting-edge, extend the boundaries of biological knowledge, and will positively impact human health. We actively engage in and are highly supportive of grant-writing activities aimed at securing funding for high-throughput genomic studies utilizing the advanced genomics technologies present within our laboratory (DNA sequencing, next generation sequencing, and single cell analysis). We will work with you to provide letters of support and proof-of-concept data and will participate in collaborative grant writing upon request. Please see our website or contact StemCore’s director, Dr. Pearl Campbell, for further details regarding our services.

Research Services
  • Next Generation Sequencing
    • RNA-Seq
    • Small RNA-Seq
    • DNA-Seq
    • ChIP-Seq
    • Methyl-Seq
    • Targeted Resequencing
  • Sanger DNA Sequencing
  • Single Cell Analysis
    • 10x Genomics
    • Fluidigm C1
  • Genotyping
  • SNP Analysis
  • Bisulfite Sequencing
  • Cell sorting
  • Flow cytometry
Sectors of Application
  • Agriculture, animal science and food
  • Education
  • Environmental technologies and related services
  • Fisheries and aquaculture
  • Forestry and forest-based industries
  • Healthcare and social services
  • Life sciences, pharmaceuticals and medical equipment
  • Manufacturing and processing

Name of specialized lab

Name of equipment

Description of function

Next Generation Sequencing Facility

Illumina MiniSeq

Small-scale next generation sequencing platform useful for amplicon, targeted RNA, small RNA, targeted gene panels, and small genome sequencing. Maximum of 25M reads per run @2x150 bases

 

Illumina NextSeq 500

Mid-scale next generation sequencing platform useful for exome, transcriptome, ChIP-Seq, and targeted resequencing. Maximum of 400M reads per run @2x150 bases

 

MX 3005p (Stratagene)

Quantitative Real-time PCR instrument used for orthogonal validation of high-throughput gene expression data and quantitation of Next Generation Sequencing libraries

Single Cell Analysis Facility

Fluidigm C1 Single Cell Analysis System

Isolates and prepares individual cells for genomic analyses including DNA, transcriptome, targeted gene expression, and miRNA sequencing. Captures, washes, stains, lyses, amplifies, and harvests cDNA from 96 cells in <13 hours for gene expression analysis

 

Fluidigm Biomark HD

Performs high-throughput nanofluidics genomics analysis of single cells or bulk populations

 

10x Genomics

Single Cell Analysis for transcriptome, immunotherapy, and other applications

 

AATI Fragment Analyzer (Advanced Analytical Technologis)

Qualitive assessment of DNA, RNA, and protein

 

Qubit 3.0 Fluorometer (ThermoFisher Scientific)

Quantitative Assessment of nucleic acids and protein

DNA Sequencing Facility

3730 DNA Analyzer (Applied Biosystems)

Medium throughput capillary-based fluorescent DNA sequencing/genotyping platform

 

Tetrad (MJ Research)

Thermocycler used for nucleic acid amplification or other enzymatic manipulations

Cell Sorting Facility

MoFlo Legacy

Lasers: 355 nm, 405 nm, 488 nm, 561nm and 640 nm

 

Title

URL

StemCore Laboratories: Catalyzing genomics projects in the Nation’s Capital

http://www.ontariogenomics.ca/news-events/news/