Pathology Research Program (PRP) Laboratory

University Health Network, Toronto, Ontario
What the facility does

Histology research lab specializing in tissue processing, embedding, sectioning, cryosectioning, special stains, immunohistochemistry (IHC) and immunofluorescence (IF), and RNAscope.

Areas of expertise

The Pathology Research Program Laboratory (PRP lab) has been providing histological services for research clients both external and internal to University Health Network since 2001. We perform both routine and customizable histology services in order to meet project needs. We are a group of experienced licensed medical laboratory technologists (MLTs) working with a wide array of species, such as zebra fish, mouse, rat, rabbit, guinea pig, ferret, dog, monkey, xenografts, allografts and human samples. Our lab has optimized over 500 antibodies to date and are acknowledged in a plethora of paper and journals. 

Research services

Fee-for-service histology research lab:

  • Tissue processing
  • Paraffin embedding
  • Paraffin and frozen sectioning
  • Special staining
  • Immunohistochemistry (IHC) and immunofluorescence (IF)
  • RNAscope
Sectors of application
  • Education
  • Life sciences, pharmaceuticals and medical equipment
  • Professional and technical services (including legal services, architecture, engineering)

Equipment

Function

Histo-Tek VP1 Tissue Processor

Automated tissue processor.

Tissue Embedding Center

Used to produce paraffin embedded tissue block.

Microm HM325 Microtome

Sectioning ultrathin sections.

Leica RM2145 Microtome

Sectioning ultrathin sections.

Leica CM1950 Cryostat

Sectioning at -20° C for sectioning frozen tissue.

Thermo Scientific Microm HM550 Cryostat Microtom

Sectioning at -20° C for sectioning frozen tissue.

Tissue-Tek Glas g2-A1 Automated Glass Coverslipper

Automated glass coverslipper.