Princess Margaret Genomics Centre

University Health Network, Toronto, Ontario
What the facility does

Genomics, epigenomics and functional genomics services and technology development. Specializing in clinically relevant samples and single cell genomics analysis.

Area(s) of Expertise

The Princess Margaret Genomics Centre (PMGC) has one of the broadest technology profiles in Canada. We are dedicated to providing high quality genomics services to researchers in Canada and around the world. Our approach is to provide the right technology to explore the problem ensuring the best possible data. We are experienced technology innovators as well as service providers.  

We are the leading provider of single cell genomics technologies in the country with particular emphasis on transcriptomics and epigenomics. We specialize in working with clinically relevant samples including those from formalin-fixed paraffin-embedded samples, fine needle aspirates, frozen tissue samples, flow sorted cells, and buccal swabs.

We provide a consultative approach to help identify the best strategy to ensure our customers obtain the best possible data for their research programs.

Research Services
  • Consultation
  • Deep-sequencing/next-generation sequencing (NGS) applications and services
  • Exome-sequencing
  • RNA-sequencing
  • Single cell mRNA-seq
  • ChIP-sequencing
  • ATAC-Seq (Assay for Transposase-Accessible Chromatin with high-throughput sequencing)
  • Single cell ATAC-seq
  • Targeted resequencing
  • CRISPR library generation and sequencing
  • Low-C sequencing
  • Methylation sequencing
  • NanoString services
  • Illumina microarrays
  • Fluidigm qPCR (quantitative polymerase chain reaction)
  • Luminex® assays
  • Digital droplet PCR (polymerase chain reaction)
  • Nucleic acid sample quality assessment
Sectors of Application
  • Health care and social services
  • Life sciences, pharmaceuticals and medical equipment

Specialized lab

Equipment

Function

Deep Sequencing Lab

Illumina NovaSeq 6000, Illumina HiSeq 2500, Illumina NextSeq 500, Illumina MiSeq, Ion Torrent Proton

Deep sequencing for genomics applications including Exome, RNA, ChIP, ATAC, and Methyl Sequencing

Single Cell Lab

10X Genomics Chromium, Fluidigm C1

Dedicated facilities for capture of cells via droplet or microfluidic capture, amplification of RNA and DNA from minute samples and processing of single cell RNA-seq and ATAC-seq applications

Targeted Genomics

NanoString nCounter, Fluidigm Biomark HD, Bio-Rad QX200™, Illumina MiSeq

Equipment and facilities for targeted RNA, DNA and epigenomics applications, including quantitative analysis