The SickKids-UHN Flow Cytometry Facility at TMDT

University Health Network, Toronto, Ontario
What the facility does

Flow cytometry, mass cytometry, cell sorting and analytical flow cytometry services and instrumentation

Area(s) of Expertise

We provide access to state-of-the-art analytical flow cytometers, mass cytometers and high-speed cell sorters and cell separators. Facility staff members operate and maintain the instruments, assist researchers in protocol selection and development, and train students, post-doctoral fellows and technologists how to operate the analytical cytometers and how to use data analysis software.  In addition, we provide phospho-flow analysis, flow cytometry acquisition services and data analysis services.

The SickKids-UHN Flow Cytometry Facility also has a location at The Hospital for Sick Children’s Peter Gilgan Centre for Research and Learning (PGCRL), for which a Navigator profile can be viewed here.

Research Services

Analytical flow cytometry (assisted and unassisted), phospho-flow analysis, cell sorting, automated magnetic cell separation, CyTOF mass cytometry (analytical mass cytometry/validated mass cytometry antibodies/antibody metal labeling service), flow cytometry training, flow and mass cytometry data analysis, consultations, protocol development.

Sectors of Application
  • Agriculture, animal science and food
  • Fisheries and aquaculture
  • Forestry and forest-based industries
  • Healthcare and social services
  • Life sciences, pharmaceuticals and medical equipment
  • Ocean industries

Name of specialized lab

Name of equipment

Description of function

TMDT

BD FACSAria II (2)

11-17 colour cell sorters

 

BD FACSAria III (2)

17-colour cell sorter

 

Becton Dickinson Influx

Eight-colour cell sorter

 

Becton Dickinson LSR II (2)

14-18 colour analytical flow cytometer

 

BD FACSCanto II (2)

Eight-colour analytical flow cytometer

 

Miltenyi Biotec AutoMACS Pro

Automated magnetic cell separator