The SickKids-UHN Flow Cytometry Facility at TMDT

University Health Network, Toronto, Ontario
What the facility does

Flow cytometry, mass cytometry, cell sorting and analytical flow cytometry services and instrumentation.  The SickKids-UHN Flow Cytometry Facility also has a location at The Hospital for Sick Children’s Peter Gilgan Centre for Research and Learning (PGCRL), for which a Navigator profile can be viewed here.

Area(s) of Expertise

We provide access to state-of-the-art analytical flow cytometers, mass cytometers and high-speed cell sorters and cell separators. Facility staff members operate and maintain the instruments, assist researchers in protocol selection and development, and train students, post-doctoral fellows and technologists how to operate the analytical cytometers and how to use data analysis software.  In addition, we provide phospho-flow analysis, flow cytometry acquisition services and data analysis services.

Research Services

Analytical flow cytometry (assisted and unassisted), phospho-flow analysis, cell sorting, automated magnetic cell separation, CyTOF mass cytometry (analytical mass cytometry/validated mass cytometry antibodies/antibody metal labeling service), flow cytometry training, flow and mass cytometry data analysis, consultations, protocol development.

Sectors of Application
  • Agriculture, animal science and food
  • Fisheries and aquaculture
  • Forestry and forest-based industries
  • Health care and social services
  • Life sciences, pharmaceuticals and medical equipment
  • Ocean industries

Name of specialized lab

Name of equipment

Description of function

TMDT

BD FACSAria II (2)

11-17 colour cell sorters

 

BD FACSAria III (2)

17-colour cell sorter

 

Becton Dickinson Influx

Eight-colour cell sorter

 

Becton Dickinson LSR II (2)

14-18 colour analytical flow cytometer

 

BD FACSCanto II (2)

Eight-colour analytical flow cytometer

 

Miltenyi Biotec AutoMACS Pro

Automated magnetic cell separator