Transmission Electron Microscopy (TEM) Core Facility

University of Ottawa, Ottawa, Ontario
What the facility does

Imaging and tissue processing solutions for transmission electron microscopy: biological sampling, cellular biology, ultrastructural imaging and anatomical quantification

Areas of expertise

The Transmission Electron Microscopy (TEM) Core Facility at the University of Ottawa provides unique imaging (TEM and electron tomography) and tissue processing solutions for your research, in partnership with CHEO’s Electron Microscopy Laboratory. The TEM core is at a strategic location, on the second floor of the Roger Guindon Hall (Faculty of Medicine) between the General Campus and the CHEO. The TEM Core houses the Ottawa region’s only TEM optimized for biological samples. Our JEM-1400Plus (120 kv) features high resolution/high contrast imaging, 3D electron tomography and montaging. It is also cryo-ready for future investments. Our microscope is equipped with a top-of-the-line, ultra-fast Gatan OneView digital camera (16MP). Brightness is ensured by a Lab6 filament as emission source. There are only 6 similar machines in Canada. Take advantage of this unique resource to bring new dimensions to your research!

Research services
  • TEM capable of imaging any biological sample (properly processed and ultrathin-sectioned)
  • Spatial resolution down to few nanometers (magnifications up to to 1,000,000x at 120kv)
  • 3D reconstruction of ultrastructure with tomography (high tilt of ±70°)
  • In-house expertise available to help you plan and execute your imaging goals
  • Protocols for tissue fixation and immuno-EM labeling are in place
  • Necessary reagents will be provided for all stages of experiments
  • Training provided for use of the main instrument
  • Time slots readily available during the day
Sectors of application
  • Education
  • Life sciences, pharmaceuticals and medical equipment
EquipmentFunction
JEOL JEM-1400(Plus) series 120kV Transmission Electron MicroscopeOperates at 120kv
Gatan OneView Camera16MP digital camera, bottom mount