The Centre for the Analysis of Genome Evolution and Function (CAGEF)

University of Toronto, Toronto, Ontario
What the facility does

Promotes and facilitates interdisciplinary research in comparative, evolutionary, and functional analyses of genomes and proteomes

Area(s) of Expertise

The Centre for the Analysis of Genome Evolution and Function (CAGEF) offers comprehensive experimental design, research, and analysis services for the analysis of genomes, transcriptomes, microbiomes, and proteomes. Our state-of-the-art facility and experienced technical and bioinformatics staff provide a broad range of genomics, proteomics, and bioinformatic services, including both standard analyses, and techniques developed by our team. We have particular expertise in microbial, plant, and environmental systems.

Research Services
  • Whole genome sequencing
  • Genome analysis
  • Single nucleotide polymorphism detection
  • Transcriptome analysis
  • Small RNA analysis
  • Chromatin immunoprecipitation assays
  • Microbiome sequencing
  • Metagenomics
  • Metatranscriptomics
  • Protein molecular weight determination
  • Protein identification
  • Protein post-translational modification determination
  • Protein pull-down assays
  • Protein tandem affinity purification tagging studies
  • Detailed proteome profiling of organisms
  • Bioinformatic services
Sectors of Application
  • Agriculture, animal science and food
  • Fisheries and aquaculture
  • Forestry and forest-based industries
  • Life sciences, pharmaceuticals and medical equipment
  • Mining, minerals and metals
  • Ocean industries

Name of equipment in use

Description of function

Illumina NextSeq 500 v2 Desktop Sequencer

Whole genome sequencing, RNA sequencing, exome sequencing,  metagenomics, metatranscriptomics

Illumina MiSeq v2 Personal Sequencer

Microbiome sequencing, high throughput amplicon sequencing, small RNA sequencing, chromatin immunoprecipitation assays

LTQ XL OrbitrapTM Hybrid Ion Trap Mass Spectrometer

Protein and post-translational modification identification

Thermo Scientific EASY-nano LC™ 1200 System

Protein and post-translational modification identification

Oxford Nanopore MinION & PromethION

Long-read genome sequencing

  • Agriculture & Agri-food Canada
  • Public Health Ontario
  • Vineland Research & Innovation Centre