Centre for the Neurobiology of Stress (CNS)

University of Toronto, Toronto, Ontario
What the facility does

Neurobiology research on stress, using various molecular, cellular and neurophysiological techniques.

Areas of expertise

The Centre for the Neurobiology of Stress (CNS) is a $10-million state-of-the-art facility. The centre tackles a series of critical areas: long-term memory and consolidation; the role of stress in natural population; maternal effects and maternal programming through epigenetic changes; neuroprotective roles of heat shock (stress) proteins; neurobiology and behaviour using fear, addiction, and other paradigms to understand brain functioning and organization; molecular and cellular mechanisms underlying Parkinson's disease; maternal adversity and early life stress-on-stress response systems in the brain and in the immune system; and host/pathogen interactions at the cellular and molecular level. 

Research services
  • Fluorescence microscopy
  • Confocal microscopy
  • Spinning disk confocal microscopy
  • Total Internal Reflection Fluorescence (TIRF) microscopy
  • Multiphoton Microscopy
  • Structured illumination (Apotome)
  • Optical sectioning
  • Transmission Electron Microscopy (TEM)
  • Scanning Electron Microscopy (SEM)
  • Patch clamping for electrophysiology
  • Micro-injection
  • Flow cytometry
  • Cell sorting
  • Gel imaging
  • Image analysis
  • Cryostat
  • Microtome
  • Ultramicrotome
  • Centrifuge
  • Autoclave
  • Polymerase Chain Reaction (PCR)
  • High Performance Liquid Chromatography (HPLC)
Sectors of application
  • Education
  • Healthcare and social services
  • Life sciences, pharmaceuticals and medical equipment
EquipmentFunction
BD FACSAria IIIFlow cytometry cell sorter
BD LSRFortessaFlow cytometry cell analyzer
Quorum Wave FX spinning disk microscopeLive cell confocal imaging
Quorum TIRF microscopeTotal Internal Reflection Fluorescence (TIRF) imaging
Olympus Multiphoton MicroscopeMultiphoton Microscopy
Zeiss ApoTome microscopeOptical sectioning
VolocityImage analysis software
ImarisImage analysis software
NeurolucidaNeuroscience software
Hitachi H-7500 TEMTransmission Electron Microscopy(TEM)
Hitachi S530 SEMScanning Electron Microscopy (SEM)
Bio-Rad PharosFXFluorescence gel imaging
Bio-Rad ChemiDoc XRS+Gel imaging
PTC-200 Thermal CyclerPolymerase Chain Reaction (PCR)
Waters HPLCHigh-Performance Liquid Chromatography (HPLC)