State-of-the-art light microscopy (optical, confocal, spectral)
The Advanced BioImaging Facility (ABIF) offers 17 state-of-the-art light microscopy platforms and the training and expertise required to use the equipment to its full potential. In-depth consultations, ongoing support, image processing, image analysis and preparation of data for publication all contribute to using researchers’ time effectively, high-quality data and elevating research projects to the next level. The facility has expertise in many areas of cellular imaging, including, but not limited to, live cell imaging, High-Content Screening (HCS), Fluorescence Recovery After Photobleaching (FRAP), Fluorescence-lifetime imaging (FLIM), Fluorescence Resonance Energy Transfer (FRET), Fluorescence correlation spectroscopy (FCS), image-correlation spectroscopy, Total internal reflection fluorescence (TIRF), spectral imaging, high-content screening, multi-photon imaging, Light Sheet, Tissue Clearing, cellular-image analysis, quantitative image analysis and custom-image analysis.
Free consultations, one-on-one training, confocal microscopy, Fluorescence-lifetime imaging (FLIM), wide-field microscopy, deconvolution, live cell time lapse, multi-colour imaging, spectral imaging, tiling for high-resolution imaging of large samples, workshops, courses, tutorials, product information sessions, equipment demonstrations
- Life sciences, pharmaceuticals and medical equipment
Specialized labs and equipment
| Equipment | Function |
|---|---|
| LSM710 confocal | Spectral imaging, photomanipulation, raster image correlation spectroscopy (RICS), FRET, tiling, multi-position live cell time lapse |
| LSM710-FLIM confocal | Time-correlated photon-counting fluorescence lifetime imaging microscope |
| AxioObserver | Multi-colour epifluorescence, phase contrast, differential interference contrast , bright field, tiling, multi-position live cell time lapse |
| Axioskop | Manual upright widefield |
| High-content screening (HCS) | Live cell time lapse combined with up to five-colour wide-field imaging. From microscope slides to 384-well plate formats. Algorithms for cell cycle, translocation, vesicle counting, cell scoring, autophagy, cell health, live/dead cell assays, mitotic index, neurite outgrowth, database and file server. |
| Lightsheet Z1 | Selective plane illumination microscope, fast imaging, live or cleared sample, multi-colour |
| LSM800 confocal | Multi-colour z-stack, tiling, multi-position, time lapse |
| Total internal reflection fluorescence (TIRF)/Spinning Disk Confocal microscope | Live cell and image correlation TIRF microscopy, spinning disk confocal, fast multicolour imaging, live cell |
| LSM880-Intravital | Multiphoton excitation, slice culture imaging, thick sample imaging, intravital imaging |
| X-Clarity workstation | Clear excess lipid and organic matter from tissues or whole organs |
| Analysis Workstations | High-end up-to-date workstations imaging software for quantifying, detailing and improving imaging |