Metabolomics and Cellular Physiology Core Facility of the Centre de recherche du CHUM

Our mission is to support university and industry research teams in the fields of metabolomics and cellular physiology, by providing small-molecule quantification services. More specifically, our metabolomics services focus on quantifying metabolites in tissues, cells and biological fluids, using mass spectrometry (targeted metabolomics). Over the years, we have developed expertise in metabolic diseases such as obesity and type 2 diabetes, with simultaneous quantification of various metabolites (lipids, nucleotides, organic acids, amino acids, etc.) in different tissues. The core facility also quantifies analytes, hormones, peptides and cytokines present in plasma and serum samples by means of ELISA and AlphaLISA, as well as in tissues by immunohistochemistry. In addition, the facility has an analyser to measure glycolysis and mitochondrial respiration, as well as a high-content cell imager (PerkinElmer Operetta) to monitor cellular phenotypic changes using fluorescent dyes.