Analytical Facility for Bioactive Molecules (AFBM)

The Hospital for Sick Children, Toronto, Ontario
What the facility does

Range of services for quantitative and qualitative metabolic analysis of biological samples for the research community

Area(s) of Expertise

The Analytical Facility for Bioactive Molecules (AFBM) is a unit of the Centre for the Study of Complex Childhood Diseases (CSCCD), a CFI funded state-of-the-art-research facility created by the Physiology & Experimental Medicine (PEM) department at The Hospital for Sick Children, Toronto, Canada.

The AFBM offers a range of services for the quantitative and qualitative analysis of biological samples to the greater scientific research community.  Services include the analysis of drugs, metabolites, lipids, sugars, amino acids, and other small molecules by liquid chromatography tandem mass spectrometry (LC/MS/MS), GC/MS, and HPLC, as well as protein/protein interactions via surface plasmon resonance.  The AFBM also offers ELISA and ELISA based multiplexing assays (Luminex) for the quantitative analysis of a wide range of metabolic, endocrinological, signaling, and inflammatory biomarkers.

Operating on a Fee-For-Service format, the AFBM aims to work closely with individual researchers to ensure targeted goal oriented method development and enable comprehensive data interpretation.

Research Services

Metabolic analysis, Bioanalysis, Immunoassays, Protein-protein/drug analysis

Sectors of Application
  • Healthcare and social services
  • Life sciences, pharmaceuticals and medical equipment

Name of specialized lab

Name of equipment

Description of function

Mass spectrometry

Agilent 5975C GC/MS

 

Thermo Fisher Scientific Delta V Plus IRMS

 

Triple quadrupole mass spectrometer: Agilent 1200 HPLC system and Sciex API5500 with SelexION

 

QTRAP mass spectrometer: Agilent 1290 UHPLC system and Sciex API5500

 

Sciex API API6600 TripleTOF

 

Sciex TOF/TOFTm 5800 system

 

Analysis of small molecules, such as amino acids, fatty acids, lipids, drugs, vitamins, hormones and peptides, and a vast array of other metabolites, biomarkers and messenger molecules by LC/MS/MS, GC/MS and MALDI-TOF. Our instrument platforms and expertise allow us to develop on demand customized methods in a variety of biological matrixes, including plasma, serum, urine, tissue, cell culture, exhaled breath, etc.

 

Mass spectrometry imaging of metabolites, drugs and proteins

 

Bioanalysis

Dionex Ultimate 3000 HPLC plus fluorometric detector

 

Electrochemical detector: Agilent 1100 HPLC with ESA Coulochem III

 

ProteOnXPR36 protein interaction array system

Bioanalysis of drugs, small molecules, vitamins, catecholamines, nucleoside phosphates, etc.

 

Surface Plasmon Resonance for protein-protein, protein-drug interactions

Immunoassays

Luminex xMAP 100/200

 

Luminex MAGPIX

 

SpectraMax M2 multi-mode microplate reader

 

PolarStar Omega microplate reader

Bioanalysis of small sample volume for multiple analytes (cytokines, metabolites, hormones, etc.)

 

Bioanalysis of small sample volume for single analytes (ELISAs)

 

Bioanalysis of small sample volume for single analytes (ELISAs), luciferase reporter gene assays, fluorescence polarization protein binding studies, enzyme kinetics, DNA, RNA and protein quantitation and viability/caspase assays

Protein purification

Beckman Coulter ProteomeLab PF2D protein fractionation system

Protein fractionation

Title

URL

Analytical Facility for Bioactive Molecules

http://www.sickkids.ca/Research/CSCCD/AFBM/index.html