Proteomics and Mass Spectrometry Core Facility

Sample preparation techniques for proteomics (desalting, reduction, alkylation, digestion), protein labeling chemistry (Tandem Mass Tagging (TMT)), Stable isotope labeling with amino acids in cell culture (SILAC), Isobaric tags for relative and absolute quantitation (iTRAQ), dimethylation), protein identification by in-gel and shotgun proteomics, intact protein analysis by mass spectrometry (MS) and tandem mass spectrometry (MS/MS), quantitative proteomic profiling (label and label-free), post-translational modification analysis, targeted and discovery metabolomics/small molecules identification/quantification, two dimensional (2D) liquid analytical and preparative chromatography, custom liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) analysis, bioinformatic solutions for proteomics, metabolomics and lipidomics workflows (ProteomeDiscover, Skyline, CompoundDiscover, LipidSearch).