Proteomics and Mass Spectrometry Core Facility

Dalhousie University, Halifax, Nova Scotia
What the facility does

Mass spectrometry-based analytical laboratory providing  proteomic, metabolomic and lipidomic services in medical and biological sciences

Area(s) of Expertise

The Proteomics and Mass Spectrometry Core Facility is a sophisticated, state-of-the-art analytical laboratory with expertise in mass spectromety analyses of biological samples. We provide well-established proteomics, metabolomics and lipidomics workflows. In addition, we offer customized analysis and method development in any of these fields of research. Our Laboratory is run exclusively by skilled, PhD-level staff that offer one-on-one support to all our clients.

Research Services

Sample preparation techniques for proteomics (desalting, reduction, alkylation, digestion), protein labeling chemistry (Tandem Mass Tagging (TMT)), Stable isotope labeling with amino acids in cell culture (SILAC), Isobaric tags for relative and absolute quantitation (iTRAQ), dimethylation), protein identification by in-gel and shotgun proteomics, intact protein analysis by mass spectrometry (MS) and tandem mass spectrometry (MS/MS), quantitative proteomic profiling (label and label-free), post-translational modification analysis, targeted and discovery metabolomics/small molecules identification/quantification, two dimensional (2D) liquid analytical and preparative chromatography, custom liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) analysis, bioinformatic solutions for proteomics, metabolomics and lipidomics workflows (ProteomeDiscover, Skyline, CompoundDiscover, LipidSearch).

Sectors of Application
  • Agriculture, animal science and food
  • Education
  • Fisheries and aquaculture
  • Life sciences, pharmaceuticals and medical equipment
  • Ocean industries

Name of equipment in use

Description of function

UltiMate 3000 UHPLC (Ultra-High Performance Liquid Chromatography)-Velos Pro-Orbitrap MS/MS (Tandem Mass Spectrometry)

LC-MS/MS (Liquid Chromatography-Tandem Mass Spectrometry) system

UltiMate 3000 UHPLC-QTRAP 5500

LC-MS/MS system

SelexION-QTRAP 5500 MS/MS

Ion mobility MS

AKTA-Pure LC (Liquid Chromatograph)

LC-UV/IC system with fraction collector

Investigator ProGEST

Automated enzymatic digestor robot

Vanquish UHPLC-QExactive-Orbitrap MS/MS

LC-MS/MS system

Agilent 1100 HPLC

LC

Agilent  5977B GC (Gas Chromatography)-MSD (Mass Selective Detector) w/7693 autosampler

GC-MS

Agilent 7696 SamplePrep Workbench

Sample prep liquid dispensing robot

Agilent 1290 Infinity II UHPLC with DAD (Diode-Array Detector)

LC with DAD

Title

URL

Dalhousie Researchers help N.S. company launch eel aquaculture business

https://www.dal.ca/news/2017/08/02/dalhousie-researchers-help-ns-company-launch-eel-aquaculture-bus.html

Sex off the Beach-Assessing safe levels of hormones in gender-switching eels with LC-DMS-MS/MS

https://theanalyticalscientist.com/issues/0617/sex-off-the-beach/

Infrastructure funding will put DAL on the map for rare disease research

https://www.dal.ca/news/2015/06/01/infrastructure-funding-will-put-dal-on-the-map-for-rare-disease-.html

Revolutionary researchers contribute to the world’s medical knowledge

https://www.dal.ca/news/2018/01/30/revolutionary-researchers-contribute-to-the-worlds-medical-knowl…