Containment Level 3 Platform of the Research Institute of the MUHC

McGill University Health Centre, Montréal, Québec
What the facility does

A state-of-the-art, multi-pod facility for studies of Mycobacterium tuberculosis, influenza virus and human immunodeficiency virus (HIV)

Area(s) of Expertise

The RI-MUHC has a multi-pod Containment Level 3 (CL3) Platform for research on Mycobacterium tuberculosis, influenza virus and HIV (CL2+). The three independent pods meet the highest safety standards the Canadian Biosafety Standards and Guidelines (PHAC and CFIA) have defined. The facility is available to RI-MUHC researchers, affiliated collaborators and external researchers whose work requires a facility where properly trained users can manipulate various pathogenic organisms. Users of the CL3 Platform must follow the established Standard Operating Procedures (SOP), as defined in the Platform's SOP manual.

Research Services

Consultation and training, including safe handling and management of tuberculosis samples, use and access to CL3 TB facility, design of microbiology, immunology, cell culture and small animal experiments, and live cell sorting, inhalation exposure studies

Sectors of Application
  • Life sciences, pharmaceuticals and medical equipment

Equipment

Function

Type II A2 safety cabinets (2)

Safe handling of pathogens

Type II B2 biological safety cabinets (6) Safe handling of pathogens

Centrifuges

 

Incubators

Stationary, shaking and rolling

Freezers and refrigerators

-20, -80°, 4°

CH Technologies Inhalation Exposure System

Acute, sub-chronic and chronic inhalation studies

 

Plas Labs anaerobic glove box

Hypoxia chamber provides controlled temperature, oxygen and carbon dioxide while maintaining a slight negative pressure

 BTX™ electroporator

Transfection of living tissues

Thermo FastPrep ® cell disrupter

DNA-RNA protein isolation

Cryostat

Well-suited for generation of high-quality frozen tissue sections

BC Accuri™ C6 (immunophenotyping platform)

Compact cytometer making flow cytometry more accessible. Easy workflows for popular applications

BD FACSAria™ Fusion Sorter (immunophenotyping platform

High-speed sorter with a fixed-alignment cuvette flow cell in a biosafety cabinet. Provides multicolour analysis.

TECAN spectrophotometer

Multimode microplate readers with access to a full range of detection methods

  • McGill University