Containment Level 3 Platform of the Research Institute of the MUHC

McGill University Health Centre, Montréal, Québec
What the facility does

A state-of-the-art, multi-pod facility for studies of Mycobacterium tuberculosis, influenza virus and human immunodeficiency virus (HIV).

Area(s) of Expertise

The RI-MUHC has a multi-pod Containment Level 3 (CL3) Platform for research on Mycobacterium tuberculosis, influenza virus and HIV (CL2+). The three independent pods meet the highest safety standards the Canadian Biosafety Standards and Guidelines (PHAC and CFIA) have defined. The facility is available to RI-MUHC researchers, affiliated collaborators and external researchers whose work requires a facility where properly trained users can manipulate various pathogenic organisms. Users of the CL3 Platform must follow the established Standard Operating Procedures (SOP), as defined in the Platform's SOP manual.

Research Services

Consultation and training, including  safe handling and management of tuberculosis samples,  use and access to CL3 TB facility, design of microbiology, immunology, cell culture and small animal experiments, and live cell sorting, inhalation exposure studies

Sectors of Application
  • Life sciences, pharmaceuticals and medical equipment

Name of equipment in use

Description of function

 Type II A2 safety cabinets (2)/ Type II B2 biological safety cabinets (6)

Safe handling of pathogens

Centrifuges

 

Incubators (stationary, shaking and rolling)

 

Freezers and refrigerators (-20, -80°, 4°)

 

CH Technologies Inhalation Exposure System

Acute, sub-chronic and chronic inhalation studies.

 

Plas Labs anaerobic glove box

Hypoxia chamber provides  controlled temperature, oxygen and carbon dioxide while maintaining a slight negative

pressure.

 BTX™ electroporator

Transfection of living tissues.

Thermo FastPrep ® cell disrupter

DNA-RNA protein isolation

Cryostat

Well-suited for generation of high- quality frozen tissue sections.

BC Accuri ™ C6 (immunophenotyping platform)

Compact cytometer making flow cytometry more accessible. Easy workflows for popular applications.

BD FACSAria ™ Fusion Sorter (immunophenotyping platform

High-speed sorter with a fixed-alignment cuvette flow cell in a biosafety cabinet. Provides multicolour analysis.

TECAN spectrophotometer

Multimode microplate readers with access to a full range of detection methods.

  • McGill University