Human retrovirology laboratory

Université du Québec à Montréal, Montréal, Québec
What the facility does

Applied human retrovirology and retroviral immunology.

Area(s) of Expertise

Dr. Barbeau’s human retrovirology lab, located in UQAM’s biological sciences department, offers a unique environment where research currently focuses on the study of human retroviruses in a pathogenic context and the context of normal development of the placenta. The level 3 containment laboratory is the only one of its kind at UQAM. It enables a wide array of experimental approaches for studying human retroviruses, including HIV. The lab is equipped primarily for studies of various types of cellular infections (primary cells and strains) and allows a variety of measurements of the replication efficiency of these viruses. Specialized equipment is dedicated to cellular transfection approaches. The CL3 laboratory is also available to Dr. Mohammad-Ali Jenabian. His research focuses on the immune response to HIV and SIV, and he uses the CL3 laboratory to handle samples from HIV-infected patents and SIV-infected monkeys.

Research Services

Measuring of viral replication, viral inhibitor tests, immune response, viral immunology, transfection of expression vectors in different types of eukaryotic cells

Sectors of Application
  • Health care and social services
  • Life sciences, pharmaceuticals and medical equipment

Name of equipment

Description of function

Beta Star 202038-DSA-EHR double-door autoclave

Decontamination and sterilization

Baker SG403A biological safety cabinets

Cell culture and infection work

Sanyo CO2 incubators

Maintaining of cell cultures

Olympus IX71 inverted microscope with digital camera and image analysis system

Visualization of cells in culture and imaging

Barnstead CY50965 nitrogen container

Cell freezing

BioTek ELx405 96-well microplate washer

96-well test instrument

Elx808 microplate reader

96-well test instrument

Gilson GF-F167300 pipette kits (4), Gilson GF-F144801 P2 pipette, GF-F21043 12x300 ml Ultra pipettes, Multispeed XP 40000101 automatic pipettes, Repeater Pro 22-46-117 pipette

Pipettes for different volumes

Sanyo MDFU-72V –86°C upright freezer, Woods R17WBE 17-cubic-foot upright refrigerator, Woods V20WB –20°C freezer

Low-temperature storage of samples

Beckman Allegra X-12R refrigerated 24-tube table microcentrifuge

Centrifuging of material of different volumes

Nucleofector (Amaxa) transfection device, Gene Pulser Xcell BioRad BTX ECM830/630 electropermeabilization device

Cell transfection equipment

  • Université Montpellier 1
  • Centers for Disease Control and Prevention
  • Université du Québec à Montréal
  • Centre hospitalier universitaire mère-enfant Sainte-Justine
  • University of Montreal Hospital Research Centre
  • McGill University Health Centre
  • Université Laval
  • University of Ottawa

Title

URL

Rétrovirologie - Des pistes de recherche oubliées (Le Devoir)

http://www.ledevoir.com/societe/education/95988/retrovirologie-des-pistes-de-recherche-oubliees

VIH : un combat sans fin (Journal (I) Média)

http://www.journalimedia.uqam.ca/vih-un-combat-sans-fin/

Virus HTLV – Un cousin du VIH qui induit le cancer (Le Devoir)

http://pvsq.org/2013/06/actualites-frequence-vih-28-juin-2013-13/

Does chronic infection in retroviruses have a sense? http://ac.els-cdn.com/S0966842X15000232/1-s2.0-S0966842X15000232-main.pdf?_tid=6b88690c-ff6d-11e6-b…