Human retrovirology laboratory

Université du Québec à Montréal, Montréal, Québec
What the facility does

Applied human retrovirology and retroviral immunology.

Areas of expertise

Dr. Barbeau’s human retrovirology lab, located in UQAM’s biological sciences department, offers a unique environment where research currently focuses on the study of human retroviruses in a pathogenic context and the context of normal development of the placenta. The containment level 3 laboratory is the only one of its kind at UQAM. It enables a wide array of experimental approaches for studying human retroviruses, including HIV. The lab is equipped primarily for studies of various types of cellular infections (primary cells and strains) and allows a variety of measurements of the replication efficiency of these viruses. Specialized equipment is dedicated to cellular transfection approaches. The CL3 laboratory is also available to Dr. Mohammad-Ali Jenabian. His research focuses on the immune response to HIV and SIV, and he uses the CL3 laboratory to handle samples from HIV-infected patents and SIV-infected monkeys.

Research services

Measuring of viral replication, viral inhibitor tests, immune response, viral immunology, transfection of expression vectors in different types of eukaryotic cells.

Sectors of application
  • Healthcare and social services
  • Life sciences, pharmaceuticals and medical equipment
EquipmentFunction
Beta Star 202038-DSA-EHR double-door autoclaveDecontamination and sterilization
Baker SG403A biological safety cabinetsCell culture and infection work
Sanyo CO2 incubatorsMaintaining of cell cultures
Olympus IX71 inverted microscope with digital camera and image analysis systemVisualization of cells in culture and imaging
Barnstead CY50965 nitrogen containerCell freezing
BioTek ELx405 96-well microplate washer96-well test instrument
Elx808 microplate reader96-well test instrument
Gilson GF-F167300 pipette kits (4), Gilson GF-F144801 P2 pipette, GF-F21043 12x300 ml Ultra pipettes, Multispeed XP 40000101 automatic pipettes, Repeater Pro 22-46-117 pipettePipettes for different volumes
Sanyo MDFU-72V –86°C upright freezer, Woods R17WBE 17-cubic-foot upright refrigerator, Woods V20WB –20°C freezerLow-temperature storage of samples
Beckman Allegra X-12R refrigerated 24-tube table microcentrifugeCentrifuging of material of different volumes
Amaxa Nucleofector transfection deviceCell transfection equipment
Gene Pulser Xcell BioRad BTX ECM830/630 electropermeabilization device 
  • Université Montpellier 1
  • Centers for Disease Control and Prevention
  • Université du Québec à Montréal
  • Centre hospitalier universitaire mère-enfant Sainte-Justine
  • University of Montreal Hospital Research Centre
  • McGill University Health Centre
  • Université Laval
  • University of Ottawa