Centre for Advanced Light Microscopy (CALM)

McMaster University, Hamilton, Ontario
What the facility does

Fosters academic and industrial partnerships through access to cutting-edge light microscopy, photopatterning, and light-assisted 3D printing techniques.

Areas of expertise

The mission of McMaster’s Centre for Advanced Light Microscopy (CALM) is to support research and training in the science, engineering, and life sciences communities by providing researchers access to a hub of excellence in light microscopy, characterized by world-class instrumentation, high quality scientific research, promoting cross-disciplinary interactions among researchers and training future generations of microscopists.

Research services

Material and bioanalytical services including: Microscopy, confocal microscopy, multiphoton microscopy, super-resolution imaging, high content analysis, slide scanning, live cell imaging, time-lapsed imaging, atomic force microscopy (AFM), image data acquisition and processing/interpretation, 3D image reconstruction and image analysis, sample preparation, custom image analysis routines, large dataset analysis, user material/instrument consultation, user training/education.

Sectors of application
  • Aerospace and satellites
  • Agriculture, animal science and food
  • Chemical industries
  • Clean technology
  • Defence and security industries
  • Education
  • Energy
  • Environmental technologies and related services
  • Healthcare and social services
  • Information and communication technologies and media
  • Life sciences, pharmaceuticals and medical equipment
  • Manufacturing and processing
  • Mining, minerals and metals
  • Professional and technical services (including legal services, architecture, engineering)
Specialized labEquipmentFunction
CALM (room B160A)ThermoFisher Evos FL Auto 2 Imaging SystemFluorescence and Brightfield Widefield Microscope.
CALM (room B160A)Bruker Dimension Icon Atomic Force Microscope system with ScanAsyst®Atomic Force Microscopy (AFM).
CALM (room B160A)ZEISS PALM Laser Capture Microdissection microscopy systemLaser Microdissection Microscopy (LCM).
CALM (room B160A)NIKON A1R HD25 Inverted Confocal MicroscopeConfocal and Widefield Microscopy with live cell incubation chamber.
CALM (room B160A)ZEISS LSM 700 Inverted Confocal MicroscopeConfocal and Widefield Microscopy with live cell incubation chamber.
CALM (room B160A)NIKON A1R HD25 Upright Confocal MicroscopeConfocal microscopy.
CALM (room B160A)Thorlabs Telesto® Series Polarization-Sensitive OCT (PS-OCT) SystemOptical Coherence Tomography (OCT) Microscopy.
CALM (room B160A)Olympus Widefield MicroscopeWidefield Microscopy.
CALM (room B160GNIKON N-SIM Super-Resolution MicroscopeSuper-Resolution Microscopy (Structured Illumination Microscopy).
Faculty of Health Sciences (FHS 4H10)ThermoFisher Evos FL Auto 2 Imaging System with incubation chamberFluorescence and Brightfield Widefield Microscopy with incubation chamber.
Engineering (B122)Asylum Research MFP-3D Atomic Force Microscope (AFM)Atomic Force Microscopy (AFM).
AN Bourns Science Building (ABB room 204)Leica TIRF/PALM-STORM microscopy systemSuper Resolution Microscopy. Photo-Activation Localization Microscopy (PALM), Stochastic Optical Reconstruction Microscopy (STORM), and Total Internal Reflection Fluorescence (TIRF) Microscopy.


  • Zeiss