Bioprocessing and Nano-Enzyme Formulation Facility (BANEFF)

Université du Québec - Institut national de la recherche scientifique (INRS), Québec, Québec
What the facility does

Bioprocessing and nanoformulations to produce high-value and safe bioproducts

Areas of expertise

The Bioprocessing and Nano-Enzyme Formulation Facility (BANEFF) works in close collaboration with universities and the industrial sector for the testing and commercialization of different technologies of bioproduction, such as enzymes, biohydrogen, butanol, citric acid and fumaric acid. In addition to the regular laboratory equipment for specific uses and with supportive analytical facilities, BANEFF comprises principally three phases: fermentation, extraction and formulation. The facilities are accessible to universities, industries and government laboratories. The three-phase installations comprise a liquid-state fermenter, two solid-state fermenters, lyophilizer, incubator shaker, ultrafiltration system and nanospray dryer, along with spectrophotometer, autoclave, nanosizer and allied equipment. The installations comprise a platform for upstream bioprocessing, downstream bioprocessing and peripheral analytical equipment. There are different ways in which clients can use the BANEFF infrastructure: (1) getting complete training on the equipment and using it themselves in the laboratory; (2) through research contracts with INRS-ETE; and (3) enhancing the research and testing potential by collaborating with the professor through different research-grant mechanisms or direct research contracts.

To date, BANEFF has carried out the diverse activities through direct training and industry-university research collaborations. BANEFF has also worked in partnership to produce enzymes for the brewery industry, biopesticides, value addition of pomace into diverse enzymes, antioxidants and animal feed, enzymes for lipid extraction and green-food alternatives.

Research services

Fermentation: solid and liquid state, pre-treatment of wastes, screening of strains for high-performance production of bioproducts, extraction and purification of bioproducts, microformulation and nanoformulation of bioproducts, analysis of unknown metabolites, degradation by-products of organic compounds in the environment for complex matrices such as wastes, waste water, sludge and soils

Sectors of application
  • Agriculture, animal science and food
  • Energy
  • Environmental technologies and related services
Specialized labEquipmentFunction
Bioprocessing LaboratoryLiquid-state fermenter, including hydrogen and CO2 sensor and mechanical foam breaker and chiller unitsFor fermentation of different substrates coupled with the measurement of gases during aerobic and anaerobic fermentation
Bioprocessing LaboratorySolid-state fermenter (rotating drum type), including in situ sterilization, measurement of moisture, pH and CO2For fermentation of solid and semi-solid substrates with total solids concentration exceeding 80 g/L
Bioprocessing LaboratoryAutoclave for dry and liquid samplesSterilization of dry and wet media for pure-strain fermentation and decontamination of petris/wastes
Bioprocessing LaboratoryPortable benchtop laminar-flow chamberFor strain isolation, plating and all microbiological assays
Bioprocessing LaboratoryUltrasonication equipmentFor pretreatment and breakdown of complex organic matter
Bioprocessing LaboratoryPhase contrast microscopeTo assess contamination during fermentation and for general observation of microbial growth on petris
Bioprocessing LaboratoryPeristaltic pumpsFor transfer of different substrates and fluids into the fermenter
Bioprocessing LaboratoryNitrogen evaporatorTo concentrate and dry precious bioproduct samples under nitrogen
Downstream Bioprocessing LaboratoryUltrafiltration equipmentFor extraction and concentration of different components of fermentation broths
Downstream Bioprocessing LaboratoryUltrasonicationFor extraction of intracellular material
Downstream Bioprocessing LaboratoryLyophilizationFreeze-drying of liquid samples for pre-formulations, analysis and storage
Downstream Bioprocessing LaboratorySDS-PAGEFor data profiling of different protein components in the extracted fermentation broths
Downstream Bioprocessing LaboratoryMicrofiltrationFor filtration of different fermented broths for analysis
Downstream Bioprocessing LaboratoryNanospray dryerFor nanoformulation of different types, such as encapsulation, powder and emulsion
Peripheral Analytical LaboratoryUV-Vis spectrophotometer, including microplate readerFor analysis of microbial growth, enzymes and chemicals
Peripheral Analytical LaboratoryNanosizer, including autotitration and zeta potential measurementsFor analysis of particle size in the nanometer range
Peripheral Analytical LaboratoryWater bath for incubation experiments during analysisTo facilitate reactions during analysis
Peripheral Analytical LaboratoryAccelerated extraction system, including high pressureFor extraction of organic compounds from complex matrices, such as waste water, wastes and soils
Peripheral Analytical LaboratoryMicrowave extractionFor extraction of organic compounds in small volumes from solid and liquid media
  • La Barberie Inc.
  • Lallemand Inc.
  • Magnus Inc.
  • Aliments Breton Inc.
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