Faculty of Medicine & Dentistry Cell Imaging Centre

University of Alberta, Edmonton, Alberta
What the facility does

Offers access to, and training for, state-of-the-art quantitative light and electron microscopy

Area(s) of Expertise

The Cell Imaging Centre is a shared resource lab that provides services and training for light and electron microscopy and a wide range of cutting-edge image acquisition equipment. We consult on microscopy experimental design and offer full technical assistance – from sample processing through image analysis – on a wide range of equipment. We support experiments using basic widefield fluorescence microscopy, confocal microscopy, and total internal reflection fluorescence (TIRF) microscopy, and offer super resolution microscopy in the form of structured illumination microscopy and single molecule localisation microscopy. Additionally, we provide expertise in both scanning and transmission electron microscopy. Dynamic studies can be undertaken using live cell imaging on many of our resources.

In addition to instrument access and software services, we offer a comprehensive training program that includes theoretical training, hands-on training, and monthly workshops on advanced topics in biomedical imaging and regular commercial product demonstrations.

The Faculty of Medicine Dentistry also offers a Flow Cytometry Facility.

Research Services
  • Core staff assisted or self-use of microscopy resources, image acquisition
  • Comprehensive hands-on training for each piece of equipment
  • Regular theoretical courses
  • Advanced training for super resolution
  • Assistance with development and optimization of imaging experiments and methods
  • Assistance with experimental design
  • Software for data analysis
  • Software training and analysis assistance
  • Consistent and extensive instrumentation quality control
Sectors of Application
  • Agriculture, animal science and food
  • Healthcare and social services
  • Life sciences, pharmaceuticals and medical equipment

Equipment

Function

Spinning Disk Confocal Microscope

Excitation possible at 405nm, 440nm, 491nm, 561nm, 640nm. Yokogawa CSU-X1 spinning disc head. Hamamatsu C-9100-13 EMCCD. Volocity Acquisition software, motorized stage/microscope. Temperature/gas/humidity controlled chamber enables live cell imaging. 20/40/60 lenses. IX-81 inverted microscope.

Spinning Disk Confocal Microscope

Excitation possible at 405nm, 440nm, 491nm, 561nm, 640nm. Yokogawa CSU-10 spinning disc head. Hamamatsu C-9100-13 EMCCD. Volocity Acquisition software, motorized stage/microscope. Temperature/gas/humidity controlled chamber enables live cell imaging. 20/40/60 lenses. IX-81 inverted microscope.

Leica TCS SP5 confocal laser scanning microscope

3 standard PMTs, 2 HyD detectors. 405nm, 458nm, 476nm, 488nm, 496nm, 514nm, 543nm, 633nm excitation. AOBS system. DMI6000B inverted microscope.

GE Healthcare DeltaVision Elite deconvolution microscope

IX-71 inverted microscope, 40X/60X lenses, Solid State Illumination for standard 4-color imaging or CFP/YFP experiments. 2KX2K Edge sCMOS camera. Environmental control for live cell imaging.

Zeiss Axio Observer.Z1 inverted fluorescence microscope

LED illumination at 365, 470, 555, and 640nm for four-color experiments. HRm CCD camera. 20/40/63X lenses. Zeiss Zen for acquisition. 2.5X lens for large overviews. Color camera for histological specimen imaging.

Leica DMI6000 inverted microscope

Widefield fluorescence microscope with standard 4 color imaging, navigator functionality, Thunder image processing

GE Healthcare OMX V4 Blaze

Provides widefield deconvolution microscopy and super resolution using structured illumination microscopy. 405nm, 488nm, 561nm and 642nm excitation. Ring TIRF, 3X sCMOS cameras for fast live cell simultaneous live cell imaging. Environmental control for live cell imaging.

Hitachi S-4800 SEM with FEG

  • Scanning Electron Microscopy with Field Emission Gun
  • Resolution to 1nm at 220,000X, low and high mag modes
  • Electron gun: Cold cathode field emission type
  • Extracting vol (Vext): 0 to 6.5kV
  • Accelerating vol (Vacc): 0.5 to 30kV (in 100V steps). 3 stage electromagnetic lens, reduction type
  • Obj. lens aperture: Movable aperture (4 openings selectable/adjustable outside column), Self-cleaning thin aperture
  • Astigmatism correction coil (stigmator): Electromagnetic type
  • Scanning coil: 2 stage electronmagnetic-deflection type
  • Upper SE, high and low angle BSE, lower SE, STEM detectors

Hitachi H-7650 TEM

This transmission electron microscope (TEM) can achieve 70X to 200,000X magnification with high contrast mode at 40 to 120 kv accelerating voltage. The TEM also can achieve maximum of 500,000X magnification in the high-resolution mode. The 16-megapixel EMCCD camera mounted at the low-mount position (Advanced Microscopy Techniques) of the TEM generates very high quality images of ultrastructure.

Leica UC7 Ultramicrotome

With FC7 attachment for Cryo sectioning

Leica EM TP Auto Tissue Processor

 

Leica ACE600 Carbon Evaporator

Also sputter coater

Leica CPC Plunge Freezer

For cryo fixation and preparation