Faculty of Medicine & Dentistry Flow Cytometry Facility

University of Alberta, Edmonton, Alberta
What the facility does

Offers state-of-the-art analytical flow cytometry, cell sorting, and data analysis

Area(s) of Expertise

Flow Cytometry is the study of cells (“cytometry”) in fluid (“flow”). The technique enables multi-parametric analysis of the physical and chemical characteristics of cells at a high rate (over a 1000 cells/second). Flow Cytometry is widely used to study many aspects of cell biology, and routinely used in disease diagnosis (especially blood cancers), and in discovery and clinical research.

The University of Alberta’s Faculty of Medicine & Dentistry Flow Cytometry Facility is one of the leading image cytometry centers in both Canada and internationally. Our facility’s unique service offerings include flow cytometry analysis at Biosafety Levels 1, 2 and 3 (BSL-1, BSL-2, and BSL-3), as well as imaging cytometry and genomic cytometry. Our instruments are suited for high parameter immune-phenotyping, rare population analysis, advanced cell sorting, and small particle analysis, among others.

In addition to instrument and software services, we offer a comprehensive training program that includes theoretical training and hands-on training, and monthly workshops on advance topics in flow cytometry and regular commercial tutorials.

Research Services
  • Core staff assisted or user-run flow cytometry, sorting, and image cytometry acquisition
  • Comprehensive hands-on training for each piece of equipment
  • Regular theoretical courses
  • Advanced training for self-sorts on the sorters
  • Assistance with development and optimization of cytometric techniques and methods
  • Assistance with antibody panel design
  • Guidance in performing longitudinal and cross platform studies with regard to data acquisition
  • Software for data analysis
  • Software training and analysis assistance
  • Consistent and extensive instrumentation quality control
Sectors of Application
  • Agriculture, animal science and food
  • Fisheries and aquaculture
  • Health care and social services
  • Life sciences, pharmaceuticals and medical equipment

Equipment

Function

Attune NxT Flow Cytometer (Thermo Fisher Scientific)

4 laser (405 nm, 488 nm, 561 nm, 638 nm) instrument that uses acoustic focusing. Ideally suited for rare event analysis.

BD FACSCanto II Flow Cytometer (Becton Dickinson Biosciences)

3 laser (405 nm, 488 nm, 633 nm) in vitro diagnostic cytometer

BD LSRFortessa Cell Analyzer (Becton Dickinson Biosciences)

3 laser (405 nm, 488 nm, 633 nm) cytometer. Suited for low parameter cell analysis.

BD LSRFortessa X-20 Cell Analyzer (Becton Dickinson Biosciences)

5 laser (355 nm, 405 nm, 488 nm, 561 nm, 633 nm) cytometer. Suited for high parameter cell analysis (BSL2 instrument).

BD LSRFortessa-SORP (Special Order Research Products) Flow Cytometer (Becton Dickinson Biosciences)

5 laser (355 nm, 405 nm, 488 nm, 561 nm, 633 nm) cytometer. Suited for high parameter cell analysis.

BD FACSAria III Cell Sorter (Becton Dickinson Biosciences)

5 laser (355 nm, 405 nm, 488 nm, 561 nm, 633 nm) cell sorter (BSL1 sorter).

BD FACSAria III Cell Sorter (Becton Dickinson Biosciences)

5 laser (355 nm, 405 nm, 488 nm, 561 nm, 633 nm) cell sorter (BSL2 sorter).

ImageStreamx Mk II Imaging Flow Cytometer (Amnis, Luminex)

3 laser (405 nm, 488 nm, 642 nm) imaging cytometer.

CytoFLEX Flow Cytometer Platform (Beckman Coulter)

4 laser (405 nm, 488 nm, 561 nm, 638 nm) cytometer.  Suited for fluorescent protein analysis. (BSL3 instrument).