What the facility does
Characterize bio-molecular protein structures at the atomic resolution, obtain their 3D protein structures and their mode of interaction, and characterize high-throughput compound/fragment/DNA-encoded library screening.
Areas of expertise
The UHN Nuclear Magnetic Resonance (NMR) Core Facility is equipped with spectrometers used to characterize protein structures, dynamics and their human disease caused mutations. The core is also equipped with a spectrometer optimized for compound/fragment/DNA-encoded library screening.
The facility’s cutting-edge NMR spectrometers allow researchers to:
- Characterize bio-molecular structures at the atomic resolution with exquisite details
- Identify protein interactions with binding partners to understand their architecture
- Study protein dynamic
- Study protein post-translational modifications acting as molecular switches
- Study structure-function relationships of biological proteins to gain insight into biological processes in relation to human disease
- Screen high-throughput drug-like leads for novel therapeutic targets
- Study metabolites of interest
Research services
- Free project consultation to discuss project feasibility (including sample preparation and NMR experiments) and expert advice.
- Full service offered to researchers who may not be able to perform their own experiments.
- Training of graduates, PhD students and post-doctoral fellows to run their own NMR experiments; support for data analysis available.
- Data collection and analysis, and full report provided upon request.
- Access to equipment: The NMR spectrometers are available to internal and external researchers. The independent use of the NMR instruments is contingent upon training and approval of each individual by the NMR Core Facility Manager.
Sectors of application
- Education
- Healthcare and social services
- Life sciences, pharmaceuticals and medical equipment
Specialized labs and equipment
| Equipment | Function |
|---|---|
| Bruker 800MHz NEO Nuclear Magnetic Resonance Spectrometer with US2 magnet (2) | Characterize bio-molecular protein structures at the atomic resolution. |
| Bruker 700 MHz Avance III HD Nuclear Magnetic Resonance Spectrometer with Ascend magnet | Characterize bio-molecular protein structures at the atomic resolution. |
| Bruker 600MHz NEO Nuclear Magnetic Resonance Spectrometer with US Plus magnet | Optimized for NMR screening applications such as small-molecule high-throughput screening, fragment-based screening and DNA-encoded libraries screening. |