Nuclear Magnetic Resonance Spectroscopy - Centre for Biologics Evaluation

Health Canada, Ottawa, Ontario
What the facility does

Nuclear Magnetic Resonance (NMR) Spectroscopy in the analysis of complex biotherapeutic molecules

Area(s) of Expertise

The work of the laboratory focuses on the development of Nuclear Magnetic Resonance (NMR) based methods for the assessment of novel recombinant protein therapeutics, subsequent entry biologics (biosimilars) and polysaccharide vaccines. Other areas of expertise include the determination of the three-dimensional structure of recombinant protein therapeutics; and the application of NMR techniques to characterize glycoprotein-based therapeutics such as glyco-conjugate vaccines and monoclonal antibodies

Research Services

Researching the application of Nuclear Magnetic Resonance spectroscopy to identify and characterize structures of recombinant protein therapeutics and polysaccharide vaccines.

Sectors of Application
  • Life sciences, pharmaceuticals and medical equipment

Name of equipment in use

Description of function

AVANCE NEO 600 MHz Nuclear Magnetic Resonance spectrometer with 5 mm Triple Resonance Cryoprobe

For the investigation structure, dynamics and function of globular proteins

AVANCE HD 700 MHZ Nuclear Magnetic Resonance spectrometer with 5mm and 1.7mm Triple Resonance Cryoprobe

For researching and analysing molecular structure

5- and 10-litre bioreactors

For the production of isotopically labelled proteins

Two (2) Fast Protein Liquid Chromatography (FPLC) systems

For protein purification

  • World Health Organization
  • United States Pharmacopeia
  • US National Institute of Standards and Technology
  • US Food and Drug Administration

Title

URL

Biologics and Genetic Therapies Directorate

https://www.canada.ca/en/health-canada/corporate/about-health-canada/branches-agencies/health-produ…

Precision and robustness of 2D-NMR for structure assessment of filgrastim biosimilars. Nature Biotech

https://www.nature.com/articles/nbt.3474

 

Dodecylphosphocholine Micelles Induce Amyloid

Formation of the PrP (110-136) Peptide. PLOS ONE

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0168021

 

 

Assessment of the structure of pegylated-recombinant

protein therapeutics by the NMR fingerprint assay. J

Pharma Bio Anal

https://www.ncbi.nlm.nih.gov/pubmed/28254519

 

Assessment of the higher order structure of Humira®,

Remicade®, Avastin®, Rituxan®, Herceptin® and

Enbrel® by 2D-NMR. J Pharm Biomed Anal

https://www.ncbi.nlm.nih.gov/pubmed/30296716

 

Application of 2D-NMR with Room Temp NMR Probes for the Assessment of Higher Order Structure of Filgrastim. J Pharm Biomed Anal

https://www.ncbi.nlm.nih.gov/pubmed/28454057

 

Monitoring effects of excipients, formulation and mutations on high order structure of filgrastim by NMR. Pharm Res

https://www.ncbi.nlm.nih.gov/pubmed/26043856