Nuclear Magnetic Resonance (NMR) Spectroscopy in the analysis of complex biotherapeutic molecules
The work of the laboratory focuses on the development of Nuclear Magnetic Resonance (NMR) based methods for the assessment of novel recombinant protein therapeutics, subsequent entry biologics (biosimilars) and polysaccharide vaccines. Other areas of expertise include the determination of the three-dimensional structure of recombinant protein therapeutics; and the application of NMR techniques to characterize glycoprotein-based therapeutics such as glyco-conjugate vaccines and monoclonal antibodies
Researching the application of Nuclear Magnetic Resonance spectroscopy to identify and characterize structures of recombinant protein therapeutics and polysaccharide vaccines.
- Life sciences, pharmaceuticals and medical equipment
Specialized labs and equipment
| Equipment | Function |
|---|---|
| AVANCE NEO 600 MHz Nuclear Magnetic Resonance spectrometer with 5 mm Triple Resonance Cryoprobe | For the investigation structure, dynamics and function of globular proteins |
| AVANCE HD 700 MHZ Nuclear Magnetic Resonance spectrometer with 5mm and 1.7mm Triple Resonance Cryoprobe | For researching and analysing molecular structure |
| 5- and 10-litre bioreactors | For the production of isotopically labelled proteins |
| Fast Protein Liquid Chromatography (FPLC) systems (2) | For protein purification |
Private and public sector research partners
- World Health Organization
- United States Pharmacopeia
- US National Institute of Standards and Technology
- US Food and Drug Administration
Additional information
| Title | URL |
|---|---|
| Biologics and Genetic Therapies Directorate | https://www.canada.ca/en/health-canada/corporate/about-health-canada/branches-agencies/health-produ… |
| Precision and robustness of 2D-NMR for structure assessment of filgrastim biosimilars. Nature Biotech | https://www.nature.com/articles/nbt.3474
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Dodecylphosphocholine Micelles Induce Amyloid Formation of the PrP (110-136) Peptide. PLOS ONE | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0168021
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Assessment of the structure of pegylated-recombinant protein therapeutics by the NMR fingerprint assay. J Pharma Bio Anal | https://www.ncbi.nlm.nih.gov/pubmed/28254519
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Assessment of the higher order structure of Humira®, Remicade®, Avastin®, Rituxan®, Herceptin® and Enbrel® by 2D-NMR. J Pharm Biomed Anal | https://www.ncbi.nlm.nih.gov/pubmed/30296716
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| Application of 2D-NMR with Room Temp NMR Probes for the Assessment of Higher Order Structure of Filgrastim. J Pharm Biomed Anal | https://www.ncbi.nlm.nih.gov/pubmed/28454057
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| Monitoring effects of excipients, formulation and mutations on high order structure of filgrastim by NMR. Pharm Res | https://www.ncbi.nlm.nih.gov/pubmed/26043856
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