Research on genomic instability, cancer genetics and cell imaging
The Genomic Centre for Cancer Research and Diagnosis is a high-technology facility for digital imaging and analysis. Research at the GCCRD includes projects in molecular biology, cell biology, histopathology, and genetic diseases. The goals of the GCCRD are basic and translational research, as well as the education of students and highly qualified personnel in genomic instability, cancer genetics and imaging.
- Microscopy and imaging of live cells,
- Study of relative nuclear and cytosolic protein levels by quantitative fluorescent immuno-staining,
- Spectral Karyotyping (SKY),
- Spectral imaging,
- Comparative Genomic Hybridization (CGH), Fluorescent In Situ Hybridization (FISH) (incl. multicolour-FISH (M-FISH), multicolour-Banding (m-BANDing), quantitative-FISH (Q-FISH) ),
- Automatic slide scanning,
- Histology,
- Pathology,
- 3-Dimensional (3-D) imaging,
- Deconvolution,
- Confocal microscopy
- Laser chromosome micro-dissection,
- Super resolution imaging
- Laser micro dissection to isolate single cells or areas of tissue
- Education
- Healthcare and social services
- Information and communication technologies and media
- Life sciences, pharmaceuticals and medical equipment
Specialized labs and equipment
Equipment |
Function |
---|---|
ASI scanner |
Scanning, tissue arrays, immunofluorescence |
Deconvolution stations (2) |
2D- and 3D- FISH and immunofluorescence |
Laser microdissection station |
Laser microdissection of chromosomes, extra-chromosomal DNA, and single cells |
Live cell station (AxioVert) |
Fluorescence and brightfield imaging of live cells and quantification of fluorescent signals |
Metasystems station |
Fluorescence In Situ Hybridization (FISH), multicolour-FISH (M-FISH), karyotyping (fluorescent and bright field), multi-colour banding (mBANDing), and Comparative Genomic Hybridization (CGH) |
Pathology station |
Bright field microscopy of chromosomes, cells, and tissues |
Scanning metasystems station |
Fluorescence In Situ Hybridization (FISH), multicolour-FISH (M-FISH), karyotyping (fluorescent and bright field); multiple slide platform for metaphase, cells, and FISH signal detection |
ASI Spectral Karyotyping (SKY) station (1) |
Spectral karyotyping (SKY) of mouse and human chromosomes, FISH, CGH, CGH array, spectral FISH, G-banding, karyotyping |
Zeiss super resolution imaging station |
2D and 3D high-resolution (20 nm) fluorescence imaging, laser scanning microscopy, FRET, FRAP, RICS, PALM, SIM |
Zeiss Axio Imager Z1 microscope station Zeiss Axio Imager Z2 (2) |
2D and 3D fluorescence microscopy, FISH, Q-FISH, immunofluorescence |
Data analysis stations (6) |
Image processing, deconvolution, analysis |
MMI CellCut Plus |
UV laser & adhesive caps that allow isolation of pure cells or tissue sections from slides |
Additional information
Title |
URL |
---|---|
Nuclear remodelling of telomeres |