Histology Core Facility

University of Ottawa, Ottawa, Ontario
What the facility does

Broad histology services for research community (i.e. tissue processing, histochemistry and immunohistochemistry staining). The Histology Core Facility (HCF) is a full-service research histology laboratory. (i.e. tissue processing, histochemistry, immunohistochemistry and protocol development).

Area(s) of Expertise

The Histology Core Facility (HCF) is a full-service research histology laboratory located in the Department of Pathology and Laboratory Medicine of the University of Ottawa. The HCF serves the University and the Ottawa Region by providing histology services to faculty, researchers, clinicians, and students. The HCF provides histology service to over 70 researchers from the University of Ottawa as well as the Ottawa Hospital Research Institute (OHRI), University of Ottawa Heart Institute (UOHI), The Ottawa Hospital Cancer Centre (TOHCC), and the Children's Hospital of Eastern Ontario (CHEO). The HCF also provides service to special projects by the Public Health Agency of Canada (PHAC), Health Canada, and the National Research Council (NRC), which contribute to the University of Ottawa's goal of excellence in research.

We provide training and education with assistance with course set-up, student tours, and monthly microtome and cryostat training.

Consultation services are available to direct and coordinate research, protocol development, teaching and clinical work. Project service requests are provided on an individual basis to accommodate the specific needs of our research clients to include: protocol development, preliminary costing services, and technical protocol information. 

Research Services

The HCF is equipped with two automatic LOGOS tissue processors, microtomes to section paraffin blocks, cryostats for sectioning of frozen tissues for immunohistochemical or immunofluorescence service requests. New equipment recently added include a Thermo-VWR NX70 Cryostar™ Cryostat, a ThermoFisher SlideMate™ Slide Printer and a fully automated  Leica Bond-III. The Bond-III is able to perform automated immunohistochemistry (IHC), in-situ hybridization (ISH), and Immunofluorescence (IF). Digital whole slide scanning on the Zeiss Mirax Midi Slide scanner can scan all brightfield slides.

Sectors of Application
  • Education
  • Health care and social services
  • Life sciences, pharmaceuticals and medical equipment

Equipment

Function

Tissue processors

Tissue dehydration and infiltration into paraffin

Embedding Centre

Blocks tissues in molten paraffin and solidifies on cold plate

Automated  Microtomes

Slices paraffin blocks to produce thin slices of tissue for staining

Automated slide stainer

Programmed routine staining

Automated coverslipper

Places a coverslip onto stained slide

Thermo-VWR NX70 Cryostar™ Cryostat

Freezing microtome to cut frozen tissue blocks.

Leica Bond-III Automated immunostainer

Performs automated immunohistochemistry (IHC), in-situ hybridization (ISH), and Immunofluorescence (IF).

Upright  Brightfield microscope

 

Zeiss Mirax Midi High-resolution slide scanner; bright field and fluorescent

Scans stained slides at high resolution for subsequent analysis and publication of high-resolution pictures.

Software

Image analysis processing

  • The Ottawa Hospital Research Institute (OHRI)
  • University of Ottawa Heart Institute (UOHI)
  • The Ottawa Hospital Cancer Centre (TOHCC)
  • Children's Hospital of Eastern Ontario (CHEO)
  • the Eastern Ontario Regional Laboratory Association (EORLA)
  • Public Health Agency of Canada (PHAC)
  • Health Canada
  • the National Research Council (NRC)
  • Canadian Nuclear Labs