Gilbert Lab

University of Toronto, Toronto, Ontario
Area(s) of Expertise

Ah, the marvel of muscle! Your skeletal muscle tissue allows you to move, smile, and breathe. When you want to move, your brain sends signals via motor neurons to the neuromuscular junction where a burst of acetylcholine is released. This in turn actives a cascade of calcium within each skeletal muscle fiber invoking tiny molecular motors to crawl along cords of actin protein and produce a coordinated contraction of your muscle. As if this weren’t enough, when skeletal muscle is injured, muscle stem cells residing in the tissue are called to action to restore muscle form and function.

As we age, our ability to regenerate skeletal muscle declines and the tissue becomes weak. Loss of skeletal muscle mass and function also occurs as a result of certain genetic conditions, inactivity and diseases like cancer. The goal of our research is to restore muscle function in these diverse disease settings by harnessing the potential of muscle stem cells that reside in muscle – an endogenous repair approach. To do this, we employ tools and materials developed by our engineering colleagues to better understand how the body controls muscle stem cells, as well as to engineer culture models of human skeletal muscle tissue.

What the facility does

Fundamental research to understand adult skeletal muscle regeneration.

Research Services

Confocal imaging, adult stem cell isolation (skeletal muscle stem cells, fibro/adipogenic progenitors from murine and human skeletal muscle tissue, two-colour flow cytometry, flow cytometry data analysis, three-dimensional culture modelling of adult skeletal muscle.

Sectors of Application
  • Life sciences, pharmaceuticals and medical equipment

Name of specialized lab

Description of function

Confocal Microscope

Confocal microscope with environmental chamber

Stem Cell Isolation and Propagation System

 

  • STEMCELL Technologies Inc