The Centre for Applied Genomics

The Hospital for Sick Children, Toronto, Ontario
What the facility does

Genomics and molecular biology research and service provision

Area(s) of Expertise

Established in 1998, The Centre for Applied Genomics (TCAG) is dedicated to conducting and promoting groundbreaking research in genomics, including service and training support for academic, government, and private sector scientists worldwide. We undertake research experimentation and data analysis for more than 800 client researchers each year, drawn from all Canadian provinces and 44 additional countries. Services include informatics, sequencing, genetic analysis, cytogenomics, biobanking, statistical analysis, and comprehensive consultative project design and execution support.

Research Services

Sequencing, bioinformatics, genotyping, microarrays, cytogenomics, biobanking, genomic DNA preparation, high performance computing, databasing, statistical analysis, project consultation

Sectors of Application
  • Agriculture, animal science and food
  • Health care and social services
  • Life sciences, pharmaceuticals and medical equipment

Name of specialized lab

Name of equipment

Description of function

Sequencing Facility

High-throughput (“next-generation”) DNA sequencers

Used for a variety of applications including whole-exome and whole-genome sequencing, metagenomics research, sequencing of targeted amplicons and regions, sequencing of immunoprecipitated or methylated DNA, pathogen and small organism genome sequencing, transcriptome sequencing, and others. Includes instruments from Life Technologies and, Illumina, including the Illumina HiSeqX for whole genome sequencing.

Microarray and Genotyping Facility

Microarray Systems

Microarray systems for genome-wide or targeted analysis of genetic variability, or gene expression analysis. These include systems from Affymetrix and Illumina.

Cytogenomics Facility

Microscope Systems

Microscopes for chromosome analysis, including Spectral (SKY) karyotyping, two-, three-, and four-colour interphase or metaphase FISH, conventional GTG-band karyotyping, analysis of model organism or stem cell chromosomes, and cancer karyotyping.

Biobanking Facility

Biobanking Equipment

Laminar flow hoods and cell culture incubators for culture and banking of human blood (lymphoblast), fibroblast cells, permanent cell lines, stem cells, and non-human cells for research.

Informatics and Statistical Analysis Facility

 

 

Computing cluster and data storage architecture, used for parallel processing of genome-wide data sets including whole-exome and whole-genome sequence data, whole-genome microarray polymorphism or gene expression data, network/pathway analysis, and statistical genetics and epidemiology analysis.

Access to Compute Canada’s High Performing Computing Facility (HPF).

  • DNA Genotek Inc.
  • Life Technologies
  • Combimatrix Corporation
  • Population Diagnostics Inc
  • Compute Canada
  • more than 60 other private sector companies
  • 200 public sector institutions

Title

URL

TCAG News Articles Archive

http://www.tcag.ca/news/index.html

TCAG Events Archive (seminars, symposia, outreach)

http://www.tcag.ca/events/index.html

List of peer-reviewed publications acknowledging TCAG support and assistance

http://www.tcag.ca/acknowledgments/index.html

TCAG Fact Sheet

http://www.tcag.ca/documents/TCAG_fact_sheet.pdf