Proteomics Platform of the Research Institute of the MUHC

McGill University Health Centre, Montréal, Québec
What the facility does

Advanced instrumentation and streamlined bioinformatics to identify and quantify proteins, and to detect biomolecular changes in health and disease

Area(s) of Expertise

The Proteomics Platform is a fully equipped, state-of-the-art proteomics lab designed to support researchers and clinicians who wish to identify, characterize and quantify proteins or other macromolecules from cells, tissues or biological fluids. Our labs contain a variety of nanoflow liquid chromatography systems and mass spectrometers, a dedicated clean room for sample preparation and a full suite of informatics tools to help clients derive meaningful answers from their data. Our staff members have extensive experience in sample preparation, analytical workflows and bioinformatics data mining and visualization.

Research Services

Protein identification, including proteins from tissues, biological fluids and gels; protein characterization, including assessment of protein mutations, variations and deletions; post-transitional modifications, including detection and quantification of post-translational modifications including phosphorylation, acetylation, methylation, ubiquitylation; protein quantification, including Multiple Reaction Monitoring (MRM), and Data Independent Acquisition (DIA) and high-resolution quantification methods; and consultation, sample preparation, data interpretation and bioinformatics analysis.

Sectors of Application
  • Life sciences, pharmaceuticals and medical equipment

Name of specialized lab 

Name of equipment in use

Description of function

Proteomics

Agilent 6650 Q-TOF mass spectrometer equipped with nano-UHPLC

The 6650 Q-TOF mass spectrometer achieves femtogram-level sensitivity.

 

ThermoScientific Orbitrap Fusion Tribrid mass spectrometer with Dionex UltiMate ™ 3000 RSLCnano system

Analysis of low-abundance, high-complexity samples to identify compounds faster and to quantify them more accurately.

 

ThermoScientific TSQ Quantiva ™ Triple Quadrupole mass spectrometer with Dionex UltiMate ™ 3000 RSLCnano system

Exceeds the most stringent analytical requirements for quantitative performance with attogram-level sensitivity

  • McGill University
  • Institut de cardiologie de Montréal
  • Centre de recherche du Centre hospitalier de l’Université de Montréal (CR CHUM)