Human Pluripotent Stem Cell Facility (HPSCF)

The Ottawa Hospital, Ottawa, Ontario
Area(s) of Expertise

Established in 2011, the hESC Laboratory was specifically designed to enable research with human embryonic and induced pluripotent stem cells at the Sprott Centre for Stem Cell Research. The instruments listed below facilitate the understanding of pluripotency in human cells and elucidate mechanisms that control and direct lineage-specific differentiation.

The lab uses high-accuracy, high-resolution cell screening, marker analysis, confocal microscopy and proteomics analyses to identify, quantify and characterize cell colonies as well as single cells growing within colonies. The goal of the facility is to teach new users how to work with ESC and iPSC as well as give valuable scientific advice on how to use these cells as a tool for research.

What the facility does

Pluripotent stem cells are capable of differentiating into all the cells of the embryo proper and adult organism. Induced pluripotent stem cells (iPSCs) are created by reprogramming mature adult cells (such as skin cells). They offer a unique opportunity to dissect early human development, generate models of disease, and develop cellular or drug therapeutics that target a disease or target specific patients with a disease (i.e., personalized medicine). Thus, iPSCs are important tools in Regenerative/ Translational/ Personalized Medicine

Research Services

Confocal, Live cell imaging, Marker analysis (4-15 colours), Proteomics of iPSC colonies, Single- and small-colony cell analysis, iPSC and ESC training, reprogramming of fibroblasts as well as cell differentiation from iPSC; DNA shearing (Covaris); and qPCR

Sectors of Application
  • Life sciences, pharmaceuticals and medical equipment

Name of specialized lab

Name of equipment

Description of function

hESC Laboratory

Cellomics ThermoFisher Arrayscan VTI high-content screening system

Live cell imaging and analysis

 

Becton Dickinson (BD) LSRFortessa cell analyzer system

High-content analysis of single cells within growing colonies

 

ProteinSimple NanoPro 1000

Proteomic analyses of single cells or small numbers of cells

 

Amnis ImageStream X multispectral imaging flow cytometer

Fluorescence imaging of cells in suspension

 

Neon transfection (episomal reprogramming of fibroblasts)

 

 

Covaris

 

 

Roche Light Cycler 480