Provides training and support to research with human embryonic and induced pluripotent stem cells (iPSC)
The Human Pluripotent Stem Cell Facility is multidimensional in its reach within the scientific community. As a facility we provide:
- All the necessary infrastructure to work with and generated human pluripotent stem cell lines
- Training on how to work with human embryonic stem cells (hESCs)/induced pluripotent stem cells (iPSCs)
- Troubleshooting resources for members of the community working with stem cells
- Tools for new and experienced investigators embarking on using stem cell models
- Contract work for researchers at the University of Ottawa, its affiliates, government agencies, and other academic and private institutions
- Symposiums and workshops for trainee education
- Letters of support, budgets, and collaborative grant writing upon request
- Training on human embryonic stem cells (hESCs)/induced pluripotent stem cells (iPSCs) culture methods (harvesting, passaging, thawing and freezing)
- Training for reprogramming (differentiation, characterization)
- Perform as a service (cell culture, reprogramming, differentiation, characterization, CRISPR genome editing)
- Fibroblast extraction and expansion
- Teratoma injection and extraction
- Protocol development
- Healthcare and social services
- Life sciences, pharmaceuticals and medical equipment
Specialized labs and equipment
Equipment | Function |
---|---|
Tri-gas incubators | For culturing cells in normoxic and hypoxic conditions |
Picking hoods | For manual removal of differentiated hESCs/iPSCs and isolation of clones |
Bio-cool | Control rate freezer |
Lonza 4D-Nucleofector Core and X Unit | Transfection system used for episomal reprogramming and gene editing |
Covaris | DNA shearing device |
ProteinSimple NanoPro 1000 | Proteomic analyses of small numbers of cells |
Amnis ImageStream X multispectral imaging flow cytometer | High throughput fluorescence imaging of cells in suspension |
Private and public sector research partners
- University of Ottawa
- Ottawa Hospital Research Institute (Core Facility Reserve Fund)
- University of Ottawa Faculty of Medicine
Additional information
Title | URL |
---|---|
Reprogramming progeria fibroblasts re-establishes a normal epigenetic landscape | |
Human Pluripotent Stem Cell-Derived TSC2 - Haploinsufficient Smooth Muscle Cells Recapitulate Features of Lymphangioleiomyomatosis | |
Metformin Preconditioning of Human Induced Pluripotent Stem Cell-Derived Neural Stem Cells Promotes Their Engraftment and Improves Post-Stroke Regeneration and Recovery | |
Engineering PTEN-L for Cell-Mediated Delivery | https://www.sciencedirect.com/science/article/pii/S2329050117301225?via%3Dihub |
Human induced pluripotent stem cell-derived lung progenitor and alveolar epithelial cells attenuate hyperoxia-induced lung injury | https://www.sciencedirect.com/science/article/pii/S1465324917307028?via%3Dihub |