Beazely Lab at the School of Pharmacy

University of Waterloo, Kitchener, Ontario
What the facility does

Electrophysiology and neuropharmacology using human induced pluripotent stem cells (hiPSCs).

Areas of expertise

The Beazely Lab at the School of Pharmacy has expertise in electrophysiology, protein biochemistry, signal transduction, neurodegeneration, neurotransmitters, neurotrophic factors, receptor tyrosine kinases. We use cell lines, cultured primary murine neurons, and human induced pluripotent stem cell models.

Research services

Whole-cell electrophysiology with an expertise in NMDA (N-methyl-D-aspartate) receptor activity, multi-electrode array measurements of cultured neuronal networks, and protein biochemical analysis including protein expression, phosphorylation, trafficking, and intracellular signaling.

Sectors of application
  • Healthcare and social services
  • Life sciences, pharmaceuticals and medical equipment
Electrophysiology rigSet up for primarily isolate whole-cell recordings.
Micro-electrode array system 
Fluorescence microscopeFor qualitative immunocytochemistry and imaging.
Human induced pluripotent stem cell culture hood 
Chronic early-life social isolation affects NMDA and TrkB receptor expression in a sex-specific manner.
Amyloid-β inhibits PDGFβ receptor activation and prevents PDGF-BB-induced neuroprotection.
5-Hydroxytryptamine type 7 receptor neuroprotection against NMDA-induced excitotoxicity is PDGFβ receptor dependent.