Canadian Glycomics Network (GlycoNet)

University of Alberta, Edmonton, Alberta
What the facility does

A one-stop global destination focused on developing carbohydrate-based drugs, vaccines and diagnostics to address areas of unmet need through glycomics research.

Areas of expertise

GlycoNet is underpinned by world-leading glycomics experts and deploys emerging technologies, integrates new protocols, and creates new tools to provide state-of-the-art glycomics tools to tackle important academic and industrial problems. We drive innovations that will ultimately provide “Made-in-Canada” solutions to critical challenges in healthcare, agriculture and environmental sustainability.

Our comprehensive glycomics services include high-throughput glycan screening, glycan profiling and analysis, glycan-ligand discovery, custom carbohydrate synthesis, glycoengineering.

Our services, instruments, technologies and professional services are available to all researchers, from Canada and abroad, across academic, private, and government sectors. The facility’s scientific and technical personnel provides high-quality, high-throughput, and competitively priced products and services to all of its clients.

The economic sectors serviced by our facility extend far beyond biomedical research and biotechnology. Our unique service portfolio will also drive academic and market place innovation in agriculture, plant biotechnology, biomaterials, environment, biofuels, disease surveillance, clinical management, and many others.

Research services
  • Drug and vaccine development
  • Drug discovery
  • Carbohydrate synthesis
  • Glycan structure-function analysis
  • Glycan screening
  • Glycoengineering
Sectors of application
  • Agriculture, animal science and food
  • Chemical industries
  • Defence and security industries
  • Education
  • Healthcare and social services
  • Life sciences, pharmaceuticals and medical equipment

Equipment

Function

NMR 700 MHz spectrometer

Identification of interactions and confirmation among molecules.

NMR 600 MHz spectrometer with upgrades for solid state capabilities

Analysis of glycan contents within the whole bacterial cell.

NMR 500 MHz spectrometer with upgrades for cryoprobe

 

MALDI-TOF-TOF tandem mass spectrometer

Matrix-Assisted Laser Desorption/Ionization-Time Of Flight (MALDI-TOF) mass spectrometry. Analysis of glycoproteins.

TOF LC-MS system

Time-of-Flight Liquid Chromatography/Mass Spectrometry system. Analysis of compounds and observations of changes.

Waters Synapt G2 mass spectrometer

Determination of weak binding interactions between two compounds

FT-ICR mass spectrometer

Fourier-transform ion cyclotron resonance (FT-ICR) mass spectrometry. Assessment of differences in mass among molecules and observations of changes, down to the atomic level, in chemical reactions.

Electrospray ionization mass spectrometry (ESI-MS) instrumentation

Determining affinities of small molecules to proteins.

Surface Plasmon Resonance system

Monitoring binding events in real time without the use of labels.

Inverted fluorescence microscope with photobleaching system

Study of interaction of fluorescence protein molecules in living cells and tissues.

Inverted fluorescence microscope with TIRF excitation

Total Internal Reflection Fluorescence (TIRF) Microscopy. Investigation of cell membrane and other surface processes in a direct manner.

Solid-phase peptide synthesizer 

Synthesis of peptides by combining amino acids.

Carbohydrate library

More than 250 compounds. Library screening, drug discovery.

  • Alberta Innovates
  • VaxAlta
  • Scripps Research Institute
  • AbbVie
  • Sussex Research
  • Sanofi
  • Agriculture and Agri-Food Canada
  • Academia Sinica
  • GlycoSyn
  • 48Hour Discovery

Title

URL

Sialic acid-containing glycolipids mediate binding and viral entry of SARS-CoV-2

https://www.nature.com/articles/s41589-021-00924-1

Genetically encoded multivalent liquid glycan array displayed on M13 bacteriophage

https://www.nature.com/articles/s41589-021-00788-5

A quantitative, high-throughput method identifies protein–glycan interactions via mass spectrometry

https://www.nature.com/articles/s42003-019-0507-2

Mass spectrometry-based shotgun glycomics for discovery of natural ligands of glycan-binding proteins

https://pubs.acs.org/doi/10.1021/acs.analchem.0c02931

Characterization of ABH-subtype donor-specific antibodies in ABO-A-incompatible kidney transplantation

https://onlinelibrary.wiley.com/doi/10.1111/ajt.16712

Neuraminidases 1 and 3 trigger atherosclerosis by desialylating low-density lipoproteins and increasing their uptake by macrophages

https://www.ahajournals.org/doi/10.1161/JAHA.120.018756