Proteomics Core Facility (PCF)

What the facility does

Proteomics analyses of all biological samples.

Areas of expertise

The Proteomics Core Facility (PCF) is able to perform all types of proteomics analyses on proteins, protein mixtures, cells, tissues, and whole organisms. This includes identifying and quantifying proteins, characterizing post-translational modifications, and determining which proteins are interacting with other proteins.

Research services

The Proteomics Core Facility (PCF) offers a broad range of services, from mass confirmations to large-scale quantitative proteomic screens, along with all the associated bioinformatics. We are also open to collaborative projects on a case-by-case basis.

Some of the services we provide:

Measure the mass and identity of a protein or peptide (e.g., to confirm successful synthesis of a peptide or the purity of a recombinant protein)
Identify unknown bands from a gel
Characterize post-translational modifications on a protein
Identify the protein interacting partners of a ligand
Measure global changes in protein levels or post-translational modifications (e.g., phosphoproteomics)
Monitor the global interaction landscape of cells
Measure differences in protein expression between samples or across disease phenotypes.

Sectors of application

Agriculture, animal science and food

Fisheries and aquaculture

Healthcare and social services

Life sciences, pharmaceuticals and medical equipment

Specialized labs and equipment

Equipment	Function
Bruker timsTOF Pro 2 Mass Spectrometer	Trapped Ion Mobility Spectrometry (TIMS). Proteomic analysis of complex samples.
Bruker timsTOF SCP Mass Spectrometer	Single Cell Proteomics (SCP). Proteomic analysis of single cells.
Level II containment cage (CL2)	Level 2 containment of honey bees for infection and transgenic experiments.
Cryogenic storage system	Cell and sample preservation.
Liquid Handling Robot	To automate sample handling.
Exploris 480 Mass Spectrometer	Proteomic analysis of complex samples.
Agilent 1200 HPLC System	High-Performance Liquid Chromatography (HPLC). For high pH reversed phase (RP-HPLC) and size exclusion separation of samples.