Research on molecular mechanisms associated with cancer.
The Proteomics Core Facility at the Université de Sherbrooke offers a wide range of mass spectrometry services for the entire research community. The main applications are the identification of purified proteins, proteins in complex samples and post-translational modifications, as well as the use of quantitative proteomics methods (label-free, SILAC, iTRAQ). We offer sample preparation services including in-gel and in-solution tryptic digestion, desalting of samples using ZipTips and bioinformatics support for the identification and quantification of proteins. We also provide the necessary reagents for all stages of experiments. The platform staff makes their expertise available directly to customers for the design, execution and analysis of experiments.
Sample preparation, reduction, alkylation, tryptic digestion (in-gel and in-solution), desalting with ZipTips, protein identification, analysis by nanoLC – MS/MS on OrbiTrap, identification by MaxQuant and/or Proteome Discoverer, quantitative proteomics, label-free, SILAC, iTRAQ.
- Chemical industries
- Education
- Life sciences, pharmaceuticals and medical equipment
Specialized labs and equipment
Equipment |
Function |
---|---|
Dionex Ultimate 3000 RSLC OrbiTrap QExactive mass spectrometer |
High-pressure liquid chromatography (HPLC). |
NanoElute HPLC timsTOF Pro mass spectrometer |
High-pressure liquid chromatography (HPLC). |
PEAKS and MaxQuant software |
Computer analysis. |