Canadian Nutrient File SNAP-CAN Reference Laboratory

Health Canada, Ottawa, Ontario
What the facility does

Analysis of fat soluble vitamins in food samples and amino acid sequencing in protein

Area(s) of Expertise

The Canadian Nutrient File team, within the Nutrition Research Division, manages the Sampling and Nutrient Analysis Program for Canada (SNAP-CAN), which collects samples of priority foods and analyzes nutrient profiles for two food categories per year specifically to meet program needs of the Food Directorate of Health Canada (i.e. not a ‘fee-for- service’ laboratory).

Research Services

Access to the specified equipment to qualified analysts.

Analysis of fat soluble vitamins in food samples by Ultra-Performance Liquid Chromatography coupled to Photodiode Array and Fluorescence Detection Tandem Mass Spectrometry (UPLC-PDA-FLR-MS/MS) Waters ACQUITY UPLC-XEVO Triple Quadruple Mass Spectrometer (UPLC-XEVO TQ-MS).

The laboratory also has equipment for amino acid sequencing in protein by sequentially cleaving amino acids from the N-terminus of a pure protein or peptide.

Sectors of Application
  • Agriculture, animal science and food
  • Life sciences, pharmaceuticals and medical equipment

Equipment

Function

UPLC-PDA-FLR-MS/MS

(Waters Acquity UPLC- Xevo TQ mass spectrometer)

Ultra-Performance Liquid Chromatography coupled to Photodiode Array and Fluorescence Detection Tandem Mass Spectrometry

 

Separation and quantification of fat soluble vitamins

Protein Sequencing System

(Applied Biosystems Procise)

Automated N-terminal sequence analysis involving a series of chemical reactions that derivatize and remove one amino acid at a time from the N-terminal of purified peptides or intact proteins

Title

URL

Canadian Nutrient File

www.healthcanada.gc.ca/cnf

Canadian Nutrient File (CNF): update on Canadian Food Composition Activities               

https://www.sciencedirect.com/science/article/pii/S088915751730100X