Development and application of new mass spectrometric methods for proteomic analysis, especially of vaccines and biotherapeutic proteins including biosimilar products
The laboratory’s area of expertise is in the development and application of mass spectrometric methods to identify and characterize peptides and proteins in complex matrices especially: vaccines, stem cells, exosomes and therapeutic biologics. Employing a range of instruments, including tandem mass spectrometers, nano-ultra high pressure liquid chromatographs and cell sorters, the laboratory is also involved in the comparative proteomic analysis of complex biological materials from cells and tissues.
Comparative proteome analysis of mixtures of proteins from cell culture or tissue as well as mass spectral analysis of less complex materials such as recombinant proteins and vaccines. The bioinformatics analysis using numerous software suites is part of the analytical process.
- Life sciences, pharmaceuticals and medical equipment
Specialized labs and equipment
| Equipment | Function |
|---|---|
| Thermo Scientific NanoLC / Orbitrap Fusion Tribrid Mass spectrometer | Highly sensitive tandem mass spectrometer that includes multiple modes of ion fragmentation |
| Therm Scientific NanoLC / Orbitrap Lumos Tribrid Mass Spectrometer | Highly sensitive tandem mass spectrometer optimized for higher molecular weight ions. Includes multiple modes of ion fragmentation. |
Private and public sector research partners
- World Health Organization
- U.S. Food and Drug Administration
- Mexico Federal Commission for the Protection against Sanitary Risk
- National Institutes for Food and Drug Control (China)
Additional information
| Title | URL |
|---|---|
| Biologics and Genetic Therapies Directorate | https://www.canada.ca/en/health-canada/corporate/about-health-canada/branches-agencies/health-produ… |
| Design expression of QconCAT protein to validate Hi3 protein quantification of influenza vaccine antigens. J Proteomics | https://www.ncbi.nlm.nih.gov/pubmed/27343760 |
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Elastin Microfibril and Integrin Linked Protein Kinase Are Markers of Regenerative Function Mesenchymal Cells. Stem Cells |
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YAP-induced resistance of cancer cells to antitubulin drugs is modulated by a hippo-independent pathway. Cancer Research |
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| Applications of High-Resolution Mass Spectrometry in Drug Discovery and Development. Future Medicine | |
| Surface modifications of influenza proteins upon virus inactivation by β-propiolactone. Proteomics |
http://onlinelibrary.wiley.com/doi/10.1002/pmic.201300096/abstract |
| Identification of candidate proteomic signature to discriminate multipotent and non-multipotent stromal cells. PLOS ONE |
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0038954 |
| Simultaneous quantification of viral antigens hemagglutinin and neuraminidase in influenza vaccines by LC-MS(E). Vaccine | http://www.sciencedirect.com/science/article/pii/S0264410X12007402 |
| Quantification of protein isoforms in MSC by reductive dimethylation of lysines in intact proteins. Proteomics | |
| Strain identification of commercial influenza vaccines by mass spectrometry. Analytical Biochemistry |
http://www.sciencedirect.com/science/article/pii/S0003269710004628 |
| Mass Spectrometry following enzyme digestion reveals phosphorylation of recombinant proteins in E. coli through mechanisms involving nucleotide binding. Journal of Proteome Research | |
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A simple slot blot for the detection of virtually all subtypes of the influenza A viral hemagglutinins using universal antibodies targeting the fusion peptide. Nature Protocols |
http://www.nature.com/nprot/journal/v5/n1/full/nprot.2009.200.html |