Mass Spectrometry - Centre for Biologics Evaluation

Health Canada, Ottawa, Ontario
What the facility does

Development and application of new mass spectrometric methods for proteomic analysis, especially of vaccines and biotherapeutic proteins including biosimilar products

Area(s) of Expertise

The laboratory’s area of expertise is in the development and application of mass spectrometric methods to identify and characterize peptides and proteins in complex matrices especially: vaccines, stem cells, exosomes and therapeutic biologics. Employing a range of instruments, including tandem mass spectrometers, nano-ultra high pressure liquid chromatographs and cell sorters, the laboratory is also involved in the comparative proteomic analysis of complex biological materials from cells and tissues.

Research Services

Comparative proteome analysis of mixtures of proteins from cell culture or tissue as well as mass spectral analysis of less complex materials such as recombinant proteins and vaccines. The bioinformatics analysis using numerous software suites is part of the analytical process.

Sectors of Application
  • Life sciences, pharmaceuticals and medical equipment
Name of equipment in use Description of function
Thermo Scientific NanoLC / Orbitrap Fusion Tribrid Mass spectrometer Highly sensitive tandem mass spectrometer that includes multiple modes of ion fragmentation
Therm Scientific NanoLC / Orbitrap Lumos Tribrid Mass Spectrometer Highly sensitive tandem mass spectrometer optimized for higher molecular weight ions. Includes multiple modes of ion fragmentation.

 

  • World Health Organization
  • U.S. Food and Drug Administration
  • Mexico Federal Commission for the Protection against Sanitary Risk
  • National Institutes for Food and Drug Control (China)
Title URL
Biologics and Genetic Therapies Directorate https://www.canada.ca/en/health-canada/corporate/about-health-canada/branches-agencies/health-produ…
Design expression of QconCAT protein to validate Hi3 protein quantification of influenza vaccine antigens. J Proteomics https://www.ncbi.nlm.nih.gov/pubmed/27343760

Elastin Microfibril and Integrin Linked Protein Kinase Are Markers of Regenerative Function Mesenchymal Cells. Stem Cells

http://onlinelibrary.wiley.com/doi/10.1002/stem.2385/full

YAP-induced resistance of cancer cells to antitubulin drugs is modulated by a hippo-independent pathway. Cancer Research

http://cancerres.aacrjournals.org/content/74/16/4493.long

Applications of High-Resolution Mass Spectrometry in Drug Discovery and Development. Future Medicine

https://www.futuremedicine.com/doi/abs/10.4155/ebo.13.346

Surface modifications of influenza proteins upon virus inactivation by β-propiolactone. Proteomics

http://onlinelibrary.wiley.com/doi/10.1002/pmic.201300096/abstract

Identification of candidate proteomic signature to discriminate multipotent and non-multipotent stromal cells. PLOS ONE

http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0038954

Simultaneous quantification of viral antigens hemagglutinin and neuraminidase in influenza vaccines by LC-MS(E). Vaccine http://www.sciencedirect.com/science/article/pii/S0264410X12007402
Quantification of protein isoforms in MSC by reductive dimethylation of lysines in intact proteins. Proteomics

https://europepmc.org/abstract/med/22144135

Strain identification of commercial influenza vaccines by mass spectrometry. Analytical Biochemistry

http://www.sciencedirect.com/science/article/pii/S0003269710004628

Mass Spectrometry following enzyme digestion reveals phosphorylation of recombinant proteins in E. coli through mechanisms involving nucleotide binding. Journal of Proteome Research

http://pubs.acs.org/doi/abs/10.1021/pr9011987

A simple slot blot for the detection of virtually all subtypes of the influenza A viral hemagglutinins using universal antibodies targeting the fusion peptide. Nature Protocols

http://www.nature.com/nprot/journal/v5/n1/full/nprot.2009.200.html