Nanotechnology - Centre for Biologics Evaluation

Health Canada, Ottawa, Ontario
What the facility does

Research to determine how modifications to starting materials affect quality attributes of nanomedicines and furthering the development of nanoscale reference materials

Areas of expertise

The laboratory’s work involves adapting existing methods and developing new ones to analyze and understand sub-visible protein aggregation, performing regulatory research to understand the mechanisms by which alterations to the individual components of a nanomedicine affect its overall attributes, performing research to identify limitations regarding methodology utilized to characterize nanomedicines and performing research to identify emerging concerns regarding the use of nanotechnology/nanomedicines and to subsequently provide scientific responses to those concerns in this rapidly advancing field. The laboratory is also actively involved in the development of novel methods for nanoparticle fabrication as well as the development of nanoscale reference materials.

Research services

The laboratory is capable of nanoparticle assessment via dynamic light scattering, single nanoparticle tracking analysis and resonant mass measurement. Additionally, the laboratory can also perform protein structure/stability assessment via circular dichroism spectropolarimeter, differential scanning calorimetry and isothermal titration calorimetry. The capability to characterize and quantify sub-visible particles in therapeutic formulations via micro-flow digital imaging analysis is also within the laboratory’s expertise.

Sectors of application
  • Life sciences, pharmaceuticals and medical equipment
JASCO J-1500 Circular Dichroism SpectrophotometerFor assessing secondary and tertiary protein structure as well as protein stability
Malvern Panalytical NanoSight NS300Automated analysis of size distribution and concentration of all types of nanoparticles from 10nm to 2000nm in diameter

Malvern Panalytical Archimedes

Particle Metrology System

Uses resonant mass measurement to detect and count particles between 50 and 5000nm
Fluid Imaging Technologies FlowCam VS seriesA fluidics system that uses a flow cell and digital imaging system to characterize and count sub-visible particles
Thermal Analysis (TA) Instruments Nano ITC (Isothermal Titration Calorimeter)A highly sensitive calorimeter capable assessing protein/protein or protein/ligand interaction

International Pharmaceutical Regulators Forum (IPRF) 

Biologics and Genetic Therapies Directorate…
Nanoparticle size and efficiency affected by presence of fatty acids during albumin nanoparticle fabrication. PLOS ONE


Toxicogenomics analysis of mouse lung responses following exposure to titanium dioxide nanomaterials. Mutagenesis


pHAST (pH-Driven Aptamer Switch for Thrombin) Catch and-Release of Target Protein.

Bioconjugate Chemistry


Evaluation of Microflow Particle Analysis for Sub-Visible Particles Formulated with Opaque Vaccine Adjuvant. PLOS ONE


Determination of Supplier-to-Supplier and Lot-to-Lot Variability in Glycation of +Re Hum Ser Alb Expressed Oryza sativa. PLOS ONE


Generation of fatty acids from 1,2-dipalmitoyl-sn-glycero-3-phosphocholine/cardiolipin liposomes. J Liposome Res


Investigation of differences in thermal stability 2 recombinant human serum albumins with 1,2-dipalmitoyl-sn-glycero-3-Phos. J Phys Chem


O-linked glycosylation leads to decreased thermal stability of interferon alpha 2b measured by 2 orthogonal techniques. Pharm Res


Influence of bovine serum albumin on secondary structure of interferon alpha 2b. Biologicals


Characterization of retention and pharmacokinetic properties of liposomal nanoparticles dihydrosphingomyelin. BBA-Biomembranes


Therapeutically rates of drug release achieved by varying drug to lipid ratio in liposomal vincristine. BBA-Biomembranes