Metabolomics Laboratory

Université de Montréal, Montréal, Québec
What the facility does

Cardiac functional and metabolic phenotyping. Targeted and non-targeted metabolomics, biomarker discovery and fluxomics. 

Area(s) of Expertise

Eager to offer high quality services, our metabolomics platform allows biological samples analysis using state-of-the-art equipment. Our chromatography units coupled to mass spectrometry enable targeted and non-targeted analyses applied to various classes of metabolites (amino acids, fatty acids, organic acids, acylcarnitines, bile acids, lipidomics, hydrosoluble compounds, etc.). We also perform metabolic flux analyses based on the consumption of substrates using stable isotopes. In addition to the analyses already developed in the laboratory, we offer personalized services including evaluation of client needs and development of analytical methods applied to specific biological matrices or metabolite classes. We offer support throughout this process, from conception to data analysis, in order to provide reliable and high quality results.

Research Services

Study design, analytical method development, biological samples preparation and analysis, data processing and analysis.

Sectors of Application
  • Agriculture, animal science and food
  • Health care and social services
  • Life sciences, pharmaceuticals and medical equipment

Name of specialized lab

Name of equipment

Description of function

Metabolomics platform

Gas chromatograph coupled to mass spectrometer (2) (GC-MS)

Identification and quantification of metabolites extracted from biological samples

 

 

Liquid chromatograph coupled to a QTOF mass spectrometer (2) (LC-QTOF)

Non-targeted semi-quantitative analysis of biological samples

 

 

Liquid chromatograph coupled to a triple quadrupole mass spectrometer (2) (LC-QQQ)

Targeted semi-quantitative analysis of  metabolites extracted from biological samples

Metabolism laboratory

Ex-vivo cardiac perfusion system in the working mode (rodents)

Metabolic and functionnal phenotyping of the healthy and diseased heart by stable isotopes

  • Université de Montréal (including the Montreal Health Innovations Coordinating Center (MHICC) and the Institut de Recherches Cliniques de Montréal)
  • McGill University
  • Université de Sherbrooke
  • Génome Canada
  • Génome Québec
  • Washington University School of Medicine
  • University of Sydney
  • University of Washington
  • Université Catholique de Louvain
  • University of Alabama at Birmingham
  • University of Alberta
  • Massachusetts General Hospital
  • Sanford-Burnham Medical Research Institute
  • Servier
  • BioMérieux

Title

URL

Circulating 4-hydroxy-nonenal-protein adducts as early non-invasive biomarkers of oxidative stress in spontaneously hypertensive rats

http://www.ncbi.nlm.nih.gov/pubmed/16781458

Prolonged QT interval and lipid alterations beyond β-oxidation in very long chain acyl-CoA dehydrogenase null mouse hearts

http://www.ncbi.nlm.nih.gov/pubmed/21685264

Metabolic effects of glutamine on the heart: Anaplerosis versus the hexosamine biosynthetic pathway

http://www.ncbi.nlm.nih.gov/pubmed/23201305

A critical perspective of 13C-isotopomer analysis by GCMS and NMR as applied to cardiac metabolism

http://www.ncbi.nlm.nih.gov/pubmed/14734255

Circulating levels of linoleic acid and HDL-cholesterol are major determinants of 4-hydroxynonenal protein adducts in patients with heart failure https://www.ncbi.nlm.nih.gov/pubmed/24494189
Validation of fatty acid intakes estimated by a food frequency questionnaire using erythrocyte fatty acid profiling in the Montreal Heart Institute Biobank https://www.ncbi.nlm.nih.gov/pubmed/25208630
Pyruvate modifies metabolic flux and nutrient sensing during extracorporeal membrane oxygenation in an immature swine model https://www.ncbi.nlm.nih.gov/pubmed/25910802
Metabolic tracing using stable isotope-labeled substrates and mass spectrometry in the perfused mouse heart https://www.ncbi.nlm.nih.gov/pubmed/26358903
MK2 deletion in mice prevents diabetes-induced perturbations in lipid metabolism and cardiac dysfunction https://www.ncbi.nlm.nih.gov/pubmed/26558681
A metabolic signature of mitochondrial dysfunction revealed through a monogenic form of Leigh syndrome https://www.ncbi.nlm.nih.gov/pubmed/26565911
SLC25A46 is required for mitochondrial lipid homeostasis and cristae maintenance and is responsible for Leigh syndrome https://www.ncbi.nlm.nih.gov/pubmed/27390132
Ivabradine and metoprolol differentially affect cardiac glucose metabolism despite similar heart rate reduction in a mouse model of dyslipidemia https://www.ncbi.nlm.nih.gov/pubmed/27496881