Metabolomics Laboratory

Université de Montréal, Montréal, Québec
What the facility does

Cardiac functional and metabolic phenotyping. Targeted and non-targeted metabolomics, biomarker discovery and fluxomics. 

Areas of expertise

Eager to offer high quality services, our metabolomics platform allows biological samples analysis using state-of-the-art equipment. Our chromatography units coupled to mass spectrometry enable targeted and non-targeted analyses applied to various classes of metabolites (amino acids, fatty acids, organic acids, acylcarnitines, bile acids, lipidomics, hydrosoluble compounds, etc.). We also perform metabolic flux analyses based on the consumption of substrates using stable isotopes. In addition to the analyses already developed in the laboratory, we offer personalized services including evaluation of client needs and development of analytical methods applied to specific biological matrices or metabolite classes. We offer support throughout this process, from conception to data analysis, in order to provide reliable and high quality results.

Research services

Study design, analytical method development, biological samples preparation and analysis, data processing and analysis.

Sectors of application
  • Agriculture, animal science and food
  • Healthcare and social services
  • Life sciences, pharmaceuticals and medical equipment
Specialized labEquipmentFunction
Metabolomics platformGas chromatograph coupled to mass spectrometer (2) (GC-MS)Identification and quantification of metabolites extracted from biological samples



Liquid chromatograph coupled to a QTOF mass spectrometer (2) (LC-QTOF)Non-targeted semi-quantitative analysis of biological samples



Liquid chromatograph coupled to a triple quadrupole mass spectrometer (2) (LC-QQQ)Targeted semi-quantitative analysis of  metabolites extracted from biological samples
Metabolism laboratoryEx-vivo cardiac perfusion system in the working mode (rodents)Metabolic and functionnal phenotyping of the healthy and diseased heart by stable isotopes
  • Université de Montréal (including the Montreal Health Innovations Coordinating Center (MHICC) and the Institut de Recherches Cliniques de Montréal)
  • McGill University
  • Université de Sherbrooke
  • Génome Canada
  • Génome Québec
  • Washington University School of Medicine
  • University of Sydney
  • University of Washington
  • Université Catholique de Louvain
  • University of Alabama at Birmingham
  • University of Alberta
  • Massachusetts General Hospital
  • Sanford-Burnham Medical Research Institute
  • Servier
  • BioMérieux
Circulating 4-hydroxy-nonenal-protein adducts as early non-invasive biomarkers of oxidative stress in spontaneously hypertensive rats
Prolonged QT interval and lipid alterations beyond β-oxidation in very long chain acyl-CoA dehydrogenase null mouse hearts
Metabolic effects of glutamine on the heart: Anaplerosis versus the hexosamine biosynthetic pathway
A critical perspective of 13C-isotopomer analysis by GCMS and NMR as applied to cardiac metabolism
Circulating levels of linoleic acid and HDL-cholesterol are major determinants of 4-hydroxynonenal protein adducts in patients with heart failure
Validation of fatty acid intakes estimated by a food frequency questionnaire using erythrocyte fatty acid profiling in the Montreal Heart Institute Biobank
Pyruvate modifies metabolic flux and nutrient sensing during extracorporeal membrane oxygenation in an immature swine model
Metabolic tracing using stable isotope-labeled substrates and mass spectrometry in the perfused mouse heart
MK2 deletion in mice prevents diabetes-induced perturbations in lipid metabolism and cardiac dysfunction
A metabolic signature of mitochondrial dysfunction revealed through a monogenic form of Leigh syndrome
SLC25A46 is required for mitochondrial lipid homeostasis and cristae maintenance and is responsible for Leigh syndrome
Ivabradine and metoprolol differentially affect cardiac glucose metabolism despite similar heart rate reduction in a mouse model of dyslipidemia