Proteomics – Mass spectrometry

Université de Sherbrooke, Sherbrooke, Québec
What the facility does

Research on molecular mechanisms associated with cancer

Area(s) of Expertise

The Proteomics Platform at the Université de Sherbrooke offers a wide range of mass spectrometry services for the entire research community. The main applications are the identification of purified proteins, proteins in complex samples and post-translational modifications, as well as the use of quantitative proteomics methods (label-free, SILAC, iTRAQ). We offer sample preparation services including in-gel and in-solution tryptic digestion, desalting of samples using ZipTips and bioinformatics support for the identification and quantification of proteins. We also provide the necessary reagents for all stages of experiments. The platform staff makes their expertise available directly to customers for the design, execution and analysis of experiments.

Research Services

Sample preparation, reduction, alkylation, tryptic digestion (in-gel and in-solution), desalting with ZipTips, protein identification, analysis by nanoLC – MS/MS on OrbiTrap, identification by MaxQuant and/or Proteome Discoverer,  quantitative proteomics, label-free, SILAC, iTRAQ

Sectors of Application
  • Chemical industries
  • Education
  • Life sciences, pharmaceuticals and medical equipment

Name of equipment

Description of function

Dionex Ultimate 3000 RSLC

OrbiTrap QExactive

HPLC and mass spectrometer

NanoElute HPLC

timsTOF Pro

HPLC and mass spectrometer
PEAKS and MaxQuant Computer analyses