RNomics Platform

Université de Sherbrooke, Sherbrooke, Québec
What the facility does

Detection and quantification of RNA and DNA in biological samples

Area(s) of Expertise

The RNomics Platform at Université de Sherbrooke provides a wide range of next-generation sequencing, PCR and bioinformatics services to the research community. Our automated computational and experimental platform has been developed to handle both large-scale sequencing and PCR-based investigations. Its primary applications are for comparative gene expression studies using RNA-seq and quantitative PCR (qPCR), and the annotation and analysis of alternative splicing using endpoint PCR coupled with microcapillary electrophoresis (ASPCR). In addition to sequencing applications, we routinely design, execute and analyze thousands of qPCR and ASPCR reactions per day. Associated services include RNA and DNA isolation and quality control from cell culture and tissue sources, as well as extensive bioinformatics support for experimental design and data analysis. Clients can access their results and all experimental data via a secure Web application, where several computational modules are available to optimize data analysis and presentation. The Platform's expert staff is also available for direct consultation at all stages of each project. The Platform is continually developing new assays and bioinformatics protocols to meet the needs of its expanding client base

Research Services

RNA and DNA extraction, RNA quality control, RNA-sequencing, next-generation sequencing, custom PCR assays, high-throughput PCR, quantitative PCR, gene expression, analysis of alternative splicing, bioinformatics, aequence data mining

Sectors of Application
  • Agriculture, animal science and food
  • Chemical industries
  • Clean technology
  • Defence and security industries
  • Energy (renewable and fossil)
  • Environmental technologies and related services
  • Fisheries and aquaculture
  • Forestry and forest-based industries
  • Healthcare and social services
  • Life sciences, pharmaceuticals and medical equipment

Name of equipment

Description of function

Agilent Bioanalyzer 2100

Microcapillary detection, quantification and QC of RNA and DNA

Caliper LC-90

Large-scale microcapillary detection and quantification of DNA (three  instruments)

Illumina MiSeq

Next generation sequencing

IonTorrent PGM

Next generation sequencing

Roche GS Junior System

Next generation sequencing

BioRad CFX384

qPCR 384-well format (three  instruments)

BioRad CFX96

qPCR 96-well format (three  instruments)

Applied Biosystems GeneAmp 9700

End-point PCR

Biomek FX

Liquid handling

Beckman GeXP Genetic Analysis System

Capillary electrophoresis